Lux chiral columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects under normal phase, reversed phase, polar organic, or SFC separation modes. Now, the addition of a seventh and eighth Lux CSP (i-Amylose-1 and i-Cellulose-5) help to provide even greater chiral separation success along with some other very useful advantages.

Lux i cellulose 5 dichlorophenylcarbamate columns hplclux i-amylose

 

lux amylose columns

When comparing all eight Lux chiral stationary phases, valuable differences can be found with the various polysaccharide supports and chiral selectors that are used. Specifically, for the new i-Cellulose-5, the dichlorophenyl-moiety portion of its selector creates a novel chiral selectivity by way of having two strong electron accepting atoms that draw the electron cloud of the phenyl ring outward. This interaction mechanism combines extremely well with the existing polar, hydrogen bonding, hydrophobic, van der Waals, and other retention mechanisms to promote better enantiomeric separations as well as to complement the other seven chlorinated and non-chlorinated Lux CSPs. Meanwhile, the 3,5-dimethylphenylcarbamate chiral selector found in the i-Amylose-1, Amylose-1, and Cellulose-1 phases is known for its broad enantioselectivity that’s produced through a combination polar and non-polar interaction mechanisms. The selectivity provided by this selector is further enhanced by its availability on two different polysaccharide supports (amylose vs cellulose) alongside an immobilized amylose version that allows for strong solvent use.

All eight Lux phases including the new immobilized i-Amylose-1 and i-Cellulose-5 are available in two different particle sizes of silica (3µm and 5µm) to aid customers with a variety of different instrumentation and needs. This allows for high efficiency analytical separations on 3µm columns to seamlessly scale to much larger 5µm Axia™ packed preparative columns for either LC or SFC. Additionally, high quality manufacturing and column packing processes were developed and implemented for all Lux media to ensure batch-to-batch and column-to-column reproducibility.

lower pressure per particle size

small particle size column conditions

Unlike other chiral columns on the market, a very distinctive feature can be found in the chemical framework of the new Lux i-Amylose-1 and i-Cellulose-5. During the manufacture of these two new chiral products, a chemical cross-linking (referred to as immobilization) between the polysaccharide and silica supports is created. This in turn provides incredibly strong solvent (THF, DCM, MtBE, Ethyl Acetate, etc.) robustness and prevents the phase from being dissolved in strong solvents. This robustness can be visualized through the two applications below where an i-Cellulose-5 column and a i-Amylose-1 column were exposed separately to a series of aggressive solvents with performance maintained throughout.

Column volumes for various solventslux8

It’s good to note that while most chromatographers may not use strong solvents routinely, this new type of robustness can be advantageous for method development and sample loading. The chemical ruggedness of Lux i-Amylose-1 and i-Cellulose-5 columns allows for the use of larger set of solvent systems which could potentially increase the success rate of chiral separations. Furthermore, with the ability to tolerate solvents such as DMSO, comes the ability to keep samples diluted in the strong organic solvents that are needed for sample solubility or maybe directly from a reaction mixture.

conditions for lux columns hplc

Additionally, this strong solvent robustness can potentially increase the levels of enantioselectivity that both i-Amylose-1 and i-Cellulose-5 can provide by allowing new strong solvent systems to be used during method development.

lux i-cellulose hplc columns for chlorpheniramine verapamil

Learn more about Lux i-Amylose-1 and i-Cellulose-5 chiral analytical and preparative LC/SFC columns at www.phenomenex.com/Lux.

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