Increase Production Economy Using Aggressive Wash Conditions

A significant cost in preparative purification processes is down time during sorbent replacement.  Sorbents are typically replaced when washing procedures no longer restore adequate performance or when backpressure remains high.  Continue Reading

The Effects of Changing Column Inner Diameter on LC-MS Applications-Part 4

Part 4: Putting Our Interpretation to the Test Guest Author: Dr. Jeff Layne Welcome back to our final installation of this blog series on the effect of column inner diameter Continue Reading

Chiral Chromatography and the Pharmaceutical Industry

chiral

Guest Author: Abraham Becerra Since its discovery, chiral chemistry has played a pivotal role in how scientists examine novel compounds. This unique characteristic describing a molecule’s geometry in three-dimensional space Continue Reading

Complementary Selectivity for Peptide Analysis

Guest Author: Dr. Jeff Layne It seems like more and more companies are shifting their focus towards the development of biopharmaceuticals these days.  Peptide analysis, whether it be intact therapeutic Continue Reading

Utilizing Mobile Phase pH to Maximize Preparative Purification Efficacy

Preparative chromatography is a specialized subset of chromatography, where the goal is to purify a target analyte (or analytes in some cases) from a complex mixture of crude sample.  The Continue Reading

Doing HPLC Method Development for Monoclonal Antibody Aggregates? Wait a “SEC”!

By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration Continue Reading