Steps to Properly Program Your Claricep Flash Column on an Rf Instrument
Having trouble getting your flash column to connect with your ISCO CombiFlash® Rf Instrument? Keep getting the same notification, “Unable to Identify RediSep Rf Column” flashing across your instrument screen? Don’t worry! This simply means you will need to do a one-time programming of your Rf instrument to use with your Claricep™ flash column.
Step 1: Installing Flash Column
Install your Claricep Flash Column on the CombiFlash Rf Instrument as seen below.
Step 2: Press “MANUAL SELECT”
As you lower the inlet port-valve to the top of the column, an on-screen message will appear matching either Figure 1 or 2. In both cases, you will click on “MANUAL SELECT” to take you to the settings menu.
Step 3: Select Media Type
At the bottom of the screen, select the menu toggle labeled “REDISEP” (Figure 3). You will then choose, “SELECT MEDIA”, which will allow you to select the type of material you will be using (for example: Silica, C18, Amine, etc.) Figure 4
Step 4: Select Column Size
Once you have chosen the type of material, you need to designate the gram size of the column. Return to the “REDISEP” tab and find the gram level that is most similar to the size of the Claricep column you want to install.
For example, the CombiFlash Rf has preset gram sizes of 4, 12, 24, 40, 80, 120, 220, and 330 grams, so for a 20g Claricep column you would select 24g as the appropriate setting.
Note: please refer to Table 1 for a comparison of corresponding Claricep and RediSep® column sizes.
Step 5: Selecting Equilibration and Flow Rate
Select the desired “EQUILIBRATION” and “FLOW RATE” as you would normally do. Different columns will have varying flow rates depending on the size, so enter the appropriate flow rate from Table 1.
Step 6: Run Length
Look for “RUN LENGTH” on the chart on the right of the instrument’s screen and select the desired length.
In the end it is easy to install a Claricep flash column on a CombiFlash Rf system once it is simply programmed. Once you have your method set-up, be sure to SAVE IT in the instrument. This allows you to run the method next time without any extra steps. You are now ready to enjoy all the benefits and cost savings that using Claricep flash columns offers.
For a more visual breakdown of this installation, check out our video “” where we take you step-by-step through the process in our lab.
For more Flash Chromatography Resources, check out below:
All you need to know about Claricep, Claricep™ Flash Brochure
Want to learn more about flash chromatography? Check out our article “What is Flash Chromatography? Discover What’s New In An Old Field”.