The Effects of Changing Column Inner Diameter on LC-MS Applications-Part 3

Part 3: What is the Best Column ID for You? Hi, and welcome back to the third installation of this article series on the effect of changing column inner diameter (ID) in chromatography.  So far, we have taken the test probe, 17-alpha-OH-progesterone, and run a simple linear gradient on the LC-MS Continue Reading

The Effects of Changing Column Inner Diameter on LC-MS Applications- Part 2

Part 2: Changing Column Inner Diameter with Constant Flow Rate Guest Author: Dr. Jeff Layne Welcome back to this article series on the effects of changing column inner diameter on LC-MS applications. Well, let’s be fair, we are using LC-MS to illustrate our discussion, but the concepts we cover apply Continue Reading

Sitting with Scientists: A Godfather of UHPLC- Dr. Jason Anspach

Meet one of the developers of UHPLC, Jason Anspach, Ph.D.- Research and Development, Senior Scientist here at Phenomenex. Ever read about advancements in science and wonder where and how it all started? When it comes to developments in Ultra High Performance Liquid Chromatography (UHPLC), Jason Anspach doesn’t have to wonder, because he was Continue Reading

The Effects of Changing Column Inner Diameter on LC-MS Applications- Part 1

Part 1: Changing Column Inner Diameter While Adjusting Flow Rate Guest Author: Dr. Jeff Layne Welcome everyone! Jeff Layne here, back with another article series, and this time we are returning to our method development roots. In this new series, we are going to address the topic of column inner Continue Reading

The Stages of Column Death!

Guest Author: Senior Scientist, Seyed Sadjadi. Congratulations! You are the proud new owner of a small stainless-steel tube packed with white sand, which will bring you fabulous results…if it lasts its life expectancy. This is the first in a series of articles to bring to light the life and death Continue Reading

Jeff Tries Cannabis Part 6: HPLC Method Development

Guest Author: Jeff Layne Well – it seems that we have a little more work to do with cannabis potency testing. I had originally planned to have the last article be the last of this HPLC method development series, however, we had a request to develop a similar separation using Continue Reading

Jeff Tries Cannabis Part 5: HPLC Method Development

Guest author: Jeff Layne Hello again, and welcome back to my cannabinoid method development article series. From the last post, we were finally able to find a few different columns that could successfully resolve all 12 of our target analytes within the pressure limitations imposed by the customer, and within Continue Reading

Jeff Tries Cannabis Part 4: Method Development

Part 4: A New Hope Guest Author: Jeff Layne Hey everyone, and welcome back to the cannabis method development series. So far in our quest to separate the twelve cannabinoids, we have messed around with gradient rates, mobile phase modifiers, and flow rates, yet have only been able to successfully Continue Reading

Sitting with Scientists: LC-MS Guru-Seyed Sadjadi

analytical scientist discussion

Meet the Guru of Liquid Chromatography and Mass Spectrometry…Senior Scientist, Seyed Sadjadi. With 26 plus years in the clinical research industry, Mr. Sadjadi is known company-wide for his insights into Liquid Chromatography and Mass Spectrometry, or LC-MS. From working as a chemist in toxicology, to field applications for LC-MS small Continue Reading

Jeff Tries Cannabis Part 3: HPLC Method Development

Effect of Flow Rate and Gradient Time in cannabis seperation phenomenex

Part 3: Effect of Flow Rate and Gradient Time Guest author: Jeff Layne Hey everybody, welcome back to my third blog article on cannabis HPLC method development. So far, we have done some preliminary gradient screening to narrow in an effective “gradient window” (75-100% acetonitrile in 10 min) and found Continue Reading