Utilizing Mobile Phase pH to Maximize Preparative Purification Efficacy

Preparative chromatography is a specialized subset of chromatography, where the goal is to purify a target analyte (or analytes in some cases) from a complex mixture of crude sample.  The ultimate goal is to purify as much target analyte as possible, in as short of a period of time as Continue Reading

Do You Know the Difference Between Viewing the Sun Normally and a Solar Eclipse?

You might not have heard anything about it, but there was a solar eclipse today. In fact, it was the first time in U.S. history that a solar eclipse traveled exclusively across America, allowing for millions of people to see the moon block out the sun on Monday, August 21st. Continue Reading

Doing HPLC Method Development for Monoclonal Antibody Aggregates? Wait a “SEC”!

By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration curve, use a standard mobile phase like 1X PBS, and voila, done and done. Of course, that isn’t the case. Continue Reading

The Effects of Changing Column Inner Diameter on LC-MS Applications-Part 3

Part 3: What is the Best Column ID for You? Hi, and welcome back to the third installation of this article series on the effect of changing column inner diameter (ID) in chromatography.  So far, we have taken the test probe, 17-alpha-OH-progesterone, and run a simple linear gradient on the LC-MS Continue Reading

Easy Installation of Phenomenex UHPLC Columns onto Waters® ACQUITY® UPLC® Systems

Installation of Phenomenex column on Acquity system

Did you know that Phenomenex LC columns are a universal fit for all HPLC and UHPLC systems on the market? It’s simple to install Phenomenex UHPLC columns onto Waters® ACQUITY® UPLC® Systems. Here’s how! 1. Disconnect the pre-column heater from the oven. 2. Separated the front and back nuts so Continue Reading

Can an UHPLC Column Have Too Much Efficiency?

Is 100% aqueous stability and unique polar selectivity not enough to convince you to try Luna Omega? Well, here is another equally-compelling reason Guest Author: Dr. Jeff Layne You Can Never Have Too Much Efficiency! The efficiency of a column is a measurement of the amount of band broadening that Continue Reading

A Comparison of Various Core-Shell C18 Phases for Method Development

When performing reversed phase LC separations, there is a natural tendency of method developers to reach for the most commonly used phase—a C18. However, there are so many different variations of C18 phases available to method developers that it often becomes difficult to choose a C18 column that one had Continue Reading

Selectivity for Polar Acids

Guest Author: Dr. Jeff Layne Is super-high efficiency and 100 % aqueous stability not enough to convince you to try Luna® Omega UHPLC Columns? Well then, how about a unique selectivity for polar acidic molecules? Selectivity for Polar Acids is Sweet! Developing robust methods for the analysis of small, polar Continue Reading