Doing HPLC Method Development for Monoclonal Antibody Aggregates? Wait a “SEC”!

By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration curve, use a standard mobile phase like 1X PBS, and voila, done and done. Of course, that isn’t the case. Continue Reading

ADCs-Magic Bullets, Failed Drugs, and Heterobifunctional Linkers

Guest Author: Brian Rivera, Product Manager: Bio Separations Over a hundred years ago, Nobel Prize laureate, Paul Ehrlich, envisioned a drug which could kill microbes but not harm the body, a “magic bullet” he called it. From mustine to monoclonal antibodies, researchers have implemented Ehrlich’s side chain theory in attempts Continue Reading