Doing HPLC Method Development for Monoclonal Antibody Aggregates? Wait a “SEC”!

By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration curve, use a standard mobile phase like 1X PBS, and voila, done and done. Of course, that isn’t the case. Continue Reading

The Science of Slushies

Earlier this week, the convenience store chain 7-Eleven, celebrated their day, July 11th, by doling out millions of free small Slurpees to their customers. However, while guzzling down your free frozen slush, did you think about the complicated chemistry that goes into making the famous cold brews? The signature frozen Continue Reading

Optimizing Sample Preparation Methods for Comprehensive Drug Research Panels from Urine

Gluca-what? Hydrolysis? Beta-something? While the terminology for urine drug testing can be confusing, the methods should not be. Improve upon traditional urine testing by learning the science behind the steps and simplifying the sample preparation. When drugs are ingested, they are tagged with a glucuronic acid during the metabolism process, Continue Reading

Rock Out to Toxicology’s Greatest Hits

This year’s hottest mix is a playlist that will rock on for centuries to come. It has been praised as the album of the year by The Rolling Chromatography* and The New York Toxicology Times* calls it a “massive hit!” Let’s go through the tracks to have a quick listen Continue Reading

Jeff Tries Cannabis Part 6: HPLC Method Development

Guest Author: Jeff Layne Well – it seems that we have a little more work to do with cannabis potency testing. I had originally planned to have the last article be the last of this HPLC method development series, however, we had a request to develop a similar separation using Continue Reading

What is USP Chapter 621?

The United States Pharmacopeia (USP) has defined the limits of “allowable adjustments” in USP General Chapter <621>, where revalidation is required if adjustment limits are exceeded. Even though this chapter might be taken as more “guidelines” than “rules”, it is best to heed the warning and take the precautions that Continue Reading

Selecting the Right Column for Your Reversed Phase Method

Selecting the right column for your reversed phase method isn’t always easy. We wanted to help in the decision-making process by breaking down the different steps you’ll need to consider when selecting your column. For a full in depth look into the selection process see our Ultimate Guide to HPLC/UHPLC Continue Reading

Jeff Tries Cannabis Part 5: HPLC Method Development

Guest author: Jeff Layne Hello again, and welcome back to my cannabinoid method development article series. From the last post, we were finally able to find a few different columns that could successfully resolve all 12 of our target analytes within the pressure limitations imposed by the customer, and within Continue Reading