The Importance of a Well Prepared Mobile Phase Explained

mobile phase buffers

Guest Author – Helen Whitby, PhD A buffer is an AQUEOUS solution containing a weak acid and its conjugate base (or a weak base and its conjugate acid). The pH of a buffer solution changes Continue Reading

HILIC Technical Tip for Stronger Analytical Methods

HILIC Technical Tip

Technical Tip: Engineering successful analytical methods using HILIC as an alternative retention mechanism Overview: This technical tip will outline hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) as an alternative retention mechanism that is particularly effective for LC-MS. Continue Reading

LC Tubing – How to Set Bend Radius with SecurityLINK

LC Tubing - How to set bend radius

Gust Author – Genevieve Hodson – Senior Technical Specialist Liquid chromatography (LC) tubing can be made from a few different types of materials depending on the needs of the laboratory. Continue Reading

Comparison of Micro LC Columns with Additional LC-MS/MS Insights

Micro LC columns

Article Written Based on Technical Application by – Roxana Eggleston-Rangel, Ryan Splitstone, and Jason Anspach, PhD. Micro LC uses columns with internal diameters (I.D.) that are typically within the range Continue Reading

Technical Tip – How to Improve Your Micro LC Column’s Performance

How to improve your Micro LC column's performance - Columna de Mico LC

Guest Author – Helen Whitby, PhD, Technical Specialist Micro LC columns offer chromatographers the opportunity to improve sensitivity or use lower flow rates due to their smaller internal diameter. The Continue Reading

Column Temperature – How to Maintain Accuracy and Stability

column temperature

Guest Author – Genevieve Hodson, Technical Specialist It is common practice to leave LC columns at ‘room temperature’, or ambient temperature conditions when running routine analytical methods. So why do Continue Reading

Synthetic Oligonucleotides – Analysis of Their Complex Structure

synthetic oligonucleotides

Guest Author – Helen Whitby, PhD., Technical Specialist Synthetic oligonucleotides are promising therapeutic agents and their characterization is fundamental to their success. However, various impurities either product-related or arising from Continue Reading

Chiral Compounds – What to Know & How They Relate to Chromatography

chiral compounds and how they relate to chromatography

Guest Author – Ryan Splitstone, Product Marketing Manager Chemical isomers originate from the same chemical formula but differ in the spatial arrangement of their chemical functional groups or bond order Continue Reading