Doing HPLC Method Development for Monoclonal Antibody Aggregates? Wait a “SEC”!

By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration curve, use a standard mobile phase like 1X PBS, and voila, done and done. Of course, that isn’t the case. Continue Reading

The Effects of Changing Column Inner Diameter on LC-MS Applications-Part 3

Part 3: What is the Best Column ID for You? Hi, and welcome back to the third installation of this article series on the effect of changing column inner diameter (ID) in chromatography.  So far, we have taken the test probe, 17-alpha-OH-progesterone, and run a simple linear gradient on the LC-MS Continue Reading

Easy Installation of Phenomenex UHPLC Columns onto Waters® ACQUITY® UPLC® Systems

Installation of Phenomenex column on Acquity system

Did you know that Phenomenex LC columns are a universal fit for all HPLC and UHPLC systems on the market? It’s simple to install Phenomenex UHPLC columns onto Waters® ACQUITY® UPLC® Systems. Here’s how! 1. Disconnect the pre-column heater from the oven. 2. Separated the front and back nuts so Continue Reading

Can an UHPLC Column Have Too Much Efficiency?

Is 100% aqueous stability and unique polar selectivity not enough to convince you to try Luna Omega? Well, here is another equally-compelling reason Guest Author: Dr. Jeff Layne You Can Never Have Too Much Efficiency! The efficiency of a column is a measurement of the amount of band broadening that Continue Reading

A Comparison of Various Core-Shell C18 Phases for Method Development

When performing reversed phase LC separations, there is a natural tendency of method developers to reach for the most commonly used phase—a C18. However, there are so many different variations of C18 phases available to method developers that it often becomes difficult to choose a C18 column that one had Continue Reading

Selectivity for Polar Acids

Guest Author: Dr. Jeff Layne Is super-high efficiency and 100 % aqueous stability not enough to convince you to try Luna® Omega UHPLC Columns? Well then, how about a unique selectivity for polar acidic molecules? Selectivity for Polar Acids is Sweet! Developing robust methods for the analysis of small, polar Continue Reading

The Effects of Changing Column Inner Diameter on LC-MS Applications- Part 2

Part 2: Changing Column Inner Diameter with Constant Flow Rate Guest Author: Dr. Jeff Layne Welcome back to this article series on the effects of changing column inner diameter on LC-MS applications. Well, let’s be fair, we are using LC-MS to illustrate our discussion, but the concepts we cover apply Continue Reading

Sitting with Scientists: A Godfather of UHPLC- Dr. Jason Anspach

Meet one of the developers of UHPLC, Jason Anspach, Ph.D.- Research and Development, Senior Scientist here at Phenomenex. Ever read about advancements in science and wonder where and how it all started? When it comes to developments in Ultra High Performance Liquid Chromatography (UHPLC), Jason Anspach doesn’t have to wonder, because he was Continue Reading

What is Core-shell and Superficially Porous Technology?

Guest Author: Technical Specialist, Ann Montross Silica media in HPLC columns were originally irregular shaped particles of silica. Then silica based HPLC columns moved to the traditionally filled, fully porous spherical particles of silica that offered improvements in chromatography over irregular shaped particles. Figure 1: Fully Porous Particles As the Continue Reading

The Effects of Changing Column Inner Diameter on LC-MS Applications- Part 1

Part 1: Changing Column Inner Diameter While Adjusting Flow Rate Guest Author: Dr. Jeff Layne Welcome everyone! Jeff Layne here, back with another article series, and this time we are returning to our method development roots. In this new series, we are going to address the topic of column inner Continue Reading