Derivatizing Reagents for GC – The Buffers Strike Back

Derivatizing Reagents

Our previous technical tip spoke about silylation reagents that may be used to derivatize a range of polar, acidic, and basic analytes to improve volatility and peak shape during gas chromatography. Silylating Continue Reading

Mycotoxins in Cannabis – Exploring More Comprehensive Testing

Mycotoxins in Cannabis

Reprinted with the kind permission of International Labmate Ltd – first printed in International Labmate,  November 2020, Volume45 Issue 7 – www.intlabmate.com The Movement Towards More Comprehensive Testing of Mycotoxins in Cannabis Guest Author Continue Reading

Derivatization in Gas Chromatography (GC) Explained

GC Derivatization tip

Gas Chromatography is a fantastic technique to achieve exceedingly sharp peaks and resolutions within a complex mixture of analytes. But each analyte must be volatile to facilitate GC analysis. Derivatization Continue Reading

The Importance of a Well Prepared Mobile Phase Explained

mobile phase buffers

Guest Author – Helen Whitby, PhD A buffer is an AQUEOUS solution containing a weak acid and its conjugate base (or a weak base and its conjugate acid). The pH of a buffer solution changes Continue Reading

HILIC Technical Tip for Stronger Analytical Methods

HILIC Technical Tip

Technical Tip: Engineering successful analytical methods using HILIC as an alternative retention mechanism Overview: This technical tip will outline hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) as an alternative retention mechanism that is particularly effective for LC-MS. Continue Reading

LC Tubing – How to Set Bend Radius with SecurityLINK

LC Tubing - How to set bend radius

Gust Author – Genevieve Hodson – Senior Technical Specialist Liquid chromatography (LC) tubing can be made from a few different types of materials depending on the needs of the laboratory. Continue Reading

Technical Tip – How to Improve Your Micro LC Column’s Performance

How to improve your Micro LC column's performance

Guest Author – Helen Whitby, PhD, Technical Specialist Micro LC columns offer chromatographers the opportunity to improve sensitivity or use lower flow rates due to their smaller internal diameter. The Continue Reading

Size Exclusion Chromatography – A Tool For Antibody Aggregate Analysis

size exclusion chromatography as a tool for antibody analysis

Guest Author – Namrata Saxena, Technical Specialist – Phenomenex Asia/Pacific Monoclonal Antibodies are large and complex biomolecules which falls in the prominent section of biotherapeutic drugs market. The complex structure Continue Reading

Nitrogen for Fast GC Analysis? Let’s Narrow Down this Idea

nitrogen and GC analysis

Guest Author – Zachary Woodward, Technical Specialist – Phenomenex USA Shorter run-times, sharper peaks, improved resolution, and low costs of carrier gas…we want it all!  These goals can seem at Continue Reading

Column Temperature – How to Maintain Accuracy and Stability

column temperature

Guest Author – Genevieve Hodson, Technical Specialist It is common practice to leave LC columns at ‘room temperature’, or ambient temperature conditions when running routine analytical methods. So why do Continue Reading