Preparative chromatography is a specialized subset of chromatography, where the goal is to purify a target analyte (or analytes in some cases) from a complex mixture of crude sample. The ultimate goal is to purify as much target analyte as possible, in as short of a period of time as Continue Reading
By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration curve, use a standard mobile phase like 1X PBS, and voila, done and done. Of course, that isn’t the case. Continue Reading
Did you know that Phenomenex LC columns are a universal fit for all HPLC and UHPLC systems on the market? It’s simple to install Phenomenex UHPLC columns onto Waters® ACQUITY® UPLC® Systems. Here’s how! 1. Disconnect the pre-column heater from the oven. 2. Separated the front and back nuts so Continue Reading
Is 100% aqueous stability and unique polar selectivity not enough to convince you to try Luna Omega? Well, here is another equally-compelling reason Guest Author: Dr. Jeff Layne You Can Never Have Too Much Efficiency! The efficiency of a column is a measurement of the amount of band broadening that Continue Reading
Guest Author: Dr. Jeff Layne Is super-high efficiency and 100 % aqueous stability not enough to convince you to try Luna® Omega UHPLC Columns? Well then, how about a unique selectivity for polar acidic molecules? Selectivity for Polar Acids is Sweet! Developing robust methods for the analysis of small, polar Continue Reading
Part 2: Changing Column Inner Diameter with Constant Flow Rate Guest Author: Dr. Jeff Layne Welcome back to this article series on the effects of changing column inner diameter on LC-MS applications. Well, let’s be fair, we are using LC-MS to illustrate our discussion, but the concepts we cover apply Continue Reading
Guest Author: Senior Scientist, Seyed Sadjadi. Congratulations! You are the proud new owner of a small stainless-steel tube packed with white sand, which will bring you fabulous results…if it lasts its life expectancy. This is the first in a series of articles to bring to light the life and death Continue Reading
The United States Pharmacopeia (USP) has defined the limits of “allowable adjustments” in USP General Chapter <621>, where revalidation is required if adjustment limits are exceeded. Even though this chapter might be taken as more “guidelines” than “rules”, it is best to heed the warning and take the precautions that Continue Reading
Meet the Guru of Liquid Chromatography and Mass Spectrometry…Senior Scientist, Seyed Sadjadi. With 26 plus years in the clinical research industry, Mr. Sadjadi is known company-wide for his insights into Liquid Chromatography and Mass Spectrometry, or LC-MS. From working as a chemist in toxicology, to field applications for LC-MS small Continue Reading
Introduction to preparative chromatography When the objective of your separation requires the isolation and purification of a valuable product, such as the case of most preparative chromatography separations, achieving the best results the first go around are not only timely but also makes financial sense. One of the formidable aspects of Continue Reading
