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pH – Why Is It Critical To Your Mobile Phase Method Development?

February 11, 2020February 11, 2020 Science UnfilteredLeave a comment

how pH can affect your mobile phase method development

Guest Author: Namrata Saxena, Technical Specialist As a chromatographer not many of us are aware of how critical pH is in your method development, sometimes it is even considered as Continue Reading

Tagged Acids,bases,ionization,Method Development,Mobile Phase,pH

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Principle HPLC Conditions for LC/MS

January 18, 2016May 16, 2017 Science UnfilteredLeave a comment

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.

Tagged Absolute Solvent Flow,Acetic,Acetic Acid,Acetonitrile,Acid,Acids,Alkylsulfonates,Analysis,Analyte,Analytes,Analyze,Buffer,Buffers,Challenge,Challenges,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatographic,Chromatography,Column,Columns,Desolvation,Detection,Eluant,Eluent,Elution,Evaporation,Flow,Flow Rate,Formic,Formic Acid,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HILIC,HPLC Column,HPLC System,HPLC Systems,Hydrophilic Interaction Liquid Chromatography,Ion,Ion-Pair Reagent,Ion-Pair Reagents,Ion-Source,Ion-Suppression Zone,Ionizable,LC,LC/MS,LC/MS/MS,Liquid Chromatography,Liquid Chromatography (LC),Mass Spec,Mass Spectral,Mass Spectrometry,Mobile Phase,Non-Volatile,Organic,Organic Acid Buffers,Parameters,Peak,Peak Shape,Peak Shapes,Peaks,pH,Phase,Phases,Phenomenex,Polar,Reversed Phase,Science,Sensitivity,Separation,Separation Sciences,Silica,Solvent,Stationary Phase,System,Systems,TEA,TFA,UHPLC,Void,Volatile,Volatile Acidic Ion-Pair Reagent,Volatile Basic Ion-Pair Reagent,Volatility

Technical Tips

Technical Tip: Protein Precipitation

August 27, 2015April 20, 2016 Science UnfilteredLeave a comment

protein precipitation

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.

Tagged Acetonitrile,Acids,Aqueous,Aqueous Based Precipitation Agent,Beverage,Bioanalytical,Chromatography,Denature,Food,Ion Exchange,Metabolites,Neutral SPE,Organic,Organic Based Precipitation Agent,Plasma,PPT,Protein Balling,Protein Precipitation,Salts,Separation Sciences,Solid Phase Extraction,Solvent,SPE,TCA,Technical Tips,Vitamin D,Zinc Sulfate

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