Milton’s Tricks, Treats, and Technical Guides

You’ve worked so hard crafting the perfect costume, decorating your home, and sneaking chocolate from your kids, we decided to treat you to the best technical guides this Halloween.

What the HILIC is HILIC?

Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.

Technical Tip: Peak Apex Flattening

Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.

How Does Temperature Affect a Compound’s Retention Time?

compound retention

The boiling point is the temperature where liquid has enough energy that molecules transferring into the vapor phase exert a pressure that is equal to the outside pressure. This allows molecules anywhere—not just the surface—to transfer to the gas phase.

Technical Tip: Principle HPLC Conditions for LC/MS

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.

Technical Note Spotlight: Neonicotinoid Analysis from Honey

Everyone knows that bees produce honey from flower nectar, but are you aware of the extensive work that goes into such a delectable creation?