Guest Author: Ramkumar Dhandapani, Ph.D.- Gas Chromatography Technical Manager In gas chromatography (GC), the separation of analytes is achieved primarily based on boiling point (Van der Waal’s interaction), although other intermolecular Continue Reading
β-Gone β-Glucuronidase Removal 96-well plates and tubes are designed to target and remove β-glucuronidase from hydrolyzed urine samples without requiring additional time or method development. In a single step and in less than 1 minute, your hydrolyzed samples are ready for analysis.
There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.
Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.
Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.
For most reversed-phase solid phase extraction applications using a silica-based SPE media, cartridges are initially conditioned using an organic solvent such as methanol, followed by rinsing with water or a buffer.
High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.
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