Backpressure Archives - SCIENCE UNFILTERED

FeaturedPharma / BiopharmaSample Preparation

Increase Production Economy Using Aggressive Wash Conditions

August 30, 2017September 7, 2017 Science UnfilteredLeave a comment

A significant cost in preparative purification processes is down time during sorbent replacement. Sorbents are typically replaced when washing procedures no longer restore adequate performance or when backpressure remains high. Continue Reading

Tagged Backpressure,chromatographic purifications,Gemini C18,Insulin,insulin purification,NaOH,Sample Prep,Sorbents

FeaturedLiquid Chromatography (LC)

Webinar Highlights – UHPLC: Small Particle Solutions for Big Chromatography Challenges

June 21, 2017August 8, 2017 Science UnfilteredLeave a comment

Senior Field Application Scientist Scott Krepich answers your questions on UHPLC column selection and shares industry-specific tips In this free webinar, Scott Krepich, Senior Field Application Scientist, Phenomenex Inc., gave Continue Reading

Tagged Backpressure,Column Chromatography,Core-Shell,Kinetex,luna omega,SelectScience,Small Particles,UHPLC

Liquid Chromatography (LC)Technical TipsUncategorized

Best and Worst Organic Solvents to Use in LC

July 14, 2016July 14, 2016 Science Unfiltered1 Comment

There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.

Tagged Acetone,Acetonitrile,Analyte,Analytes,Analytical,Analytical Chemistry,Analytical Science,Backpressure,Backpressures,Best,Chemistry,Chromatogram,Chromatographer,Chromatographers,Chromatography,Column,Column Chromatography,Column Cleaning,Columns,Elute,Elution,Ethanol,Fatty,Flow,Flow Rate,High Backpressure,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,Highly Pigmented,HPLC Column,HPLC System,Iso Propanol,LC,LC/MS/MS,Liquid Chromatography,Liquid Chromatography (LC),Methanol,n-Propanol,Organic,Organic Solvent,Organic Solvents,Peak,Peaks,Pigment,Pigmented,Polarity,Polarity Index,Protein,Proteins,Regeneration,Science,Separation,Separation Sciences,Solubility,Solubilizer,Soluble,Solvent,Solvents,Technical Tips,Tetrahydrofuran,UHPLC,UV,UV Cutoff,UV Detection,UV/Vis,Viscosity,Worst

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Split Peaks

December 17, 2015April 18, 2016 Science Unfiltered3 Comments

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.

Tagged Application,Applications,Backpressure,Backpressures,Blockage,Buffer,Buffers,Channel,Chromatography,Column,Column Lifetime,Filter,Filtered,Filtering,Flow,Flow Path,Guard Cartridge,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HPLC Column,Injection,Injections,Ionizable,LC/MS/MS,Lifetime,Liquid Chromatography,Liquid Chromatography (LC),Mobile Phase,Peak,Peaks,pH,Phenomenex,pKa,Sample,Samples,Science,Separation Sciences,Split Peak,Split Peaks,Tech Tip,Tech Tips,Technical Tip,Technical Tips,UHPLC,Void

Liquid Chromatography (LC)Technical Tips

Technical Tip: Backpressure

August 21, 2015April 20, 2016 Science UnfilteredLeave a comment

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from hardware to methodology—together.

Tagged Backpressure,Chromatography,Flow Rate,Fluctuating Backpressure,Gradient,High Backpressure,Internal Diameter,Liquid Chromatography (LC),Low Backpressure,Mobile Phase,Particle Size,Separation Sciences,Technical Tips,Troubleshooting,UHPLC

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