Our previous technical tip spoke about silylation reagents that may be used to derivatize a range of polar, acidic, and basic analytes to improve volatility and peak shape during gas chromatography. Silylating Continue Reading
The Importance of a Well Prepared Mobile Phase Explained
Guest Author – Helen Whitby, PhD A buffer is an AQUEOUS solution containing a weak acid and its conjugate base (or a weak base and its conjugate acid). The pH of a buffer solution changes Continue Reading
Buffers in Mobile Phase – Their Significance of Choosing the Correct Ones
Guest Author – Namrata Saxena, Technical Specialist We covered why pH is critical in mobile phase development, along with its effects on chromatography and analyte ionization in the first chapter Continue Reading
What the HILIC is HILIC?
Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.
Technical Tip: Bulk HPLC Sorbent Storage and Cleaning for Reuse
In the spirit of frugality, process chromatographers often want to save and store their media for future use. There are steps one can take to store bulk resins for short term and long term use, though it’s worth noting that the media will not last forever.
Poor Sample Recovery? You May Be Over-Drying
For most reversed-phase solid phase extraction applications using a silica-based SPE media, cartridges are initially conditioned using an organic solvent such as methanol, followed by rinsing with water or a buffer.
Technical Tip: Principle HPLC Conditions for LC/MS
High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.
Technical Tip: Split Peaks
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.
Ghost Peaks Technical Tip: (BOO!) How to Spot Them & Scare Them Away
Peaks in your chromatogram not attributed to the sample injected are commonly referred to as ghost peaks. Several problems can be the cause of ghost peaks manifesting, including: Late Continue Reading