Last month, Phenomenex hosted the SoCal Dietary Supplements Chromatography Forum—an all-day assembly co-sponsored by Alkemist Laboratories and US Pharmacopeia (USP) to discuss “the need and approach to modernizing USP dietary supplement monographs,” particularly those based upon chromatography.
There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.
Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.
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For most reversed-phase solid phase extraction applications using a silica-based SPE media, cartridges are initially conditioned using an organic solvent such as methanol, followed by rinsing with water or a buffer.
Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.
In the first GC columns, gas chromatography phases were simply coated onto the column. This was mostly the case with packed columns, but also the same with the very first capillary columns. Injecting water onto these phases dissolved the phase and caused the phase to elute from the columns.
High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.
Effective August 2014, the United States Pharmacopoeia and the National Formulary published the latest revision to General Chapter on Chromatography that further clarifies what “allowable adjustments” can be made to USP methods without having to revalidate these methods.
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