Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.
10 Questions You’ve Always Wanted to Ask About LC
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Poor Sample Recovery? You May Be Over-Drying
For most reversed-phase solid phase extraction applications using a silica-based SPE media, cartridges are initially conditioned using an organic solvent such as methanol, followed by rinsing with water or a buffer.
Technical Tip: Peak Apex Flattening
Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.
Technical Tip: Principle HPLC Conditions for LC/MS
High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.
Allowable Adjustments to Pharmacopoeia Methods: Simplified Version
Effective August 2014, the United States Pharmacopoeia and the National Formulary published the latest revision to General Chapter on Chromatography that further clarifies what “allowable adjustments” can be made to USP methods without having to revalidate these methods.
Technical Tip: Septum Bleed
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