Solid Phase Extraction FAQs Answered! Ashmeeta Chera, Phenomenex Sample Preparation Sales Manager and Matt Brusius, Phenomenex Sample Preparation Product Manager, discuss the major Solid Phase Extraction FAQs such as “what is Continue Reading
What is Microelution and What are the Best Strategies When Using It?
Microelution Solid Phase Extraction(SPE) is essentially miniaturized SPE. The technique is ideal for analysts that have small or limited sample volumes or for those that want to save time by Continue Reading
Best and Worst Organic Solvents to Use in LC
There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.
Technical Tip: Early Eluting Peak Shape
Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.
Poor Sample Recovery? You May Be Over-Drying
For most reversed-phase solid phase extraction applications using a silica-based SPE media, cartridges are initially conditioned using an organic solvent such as methanol, followed by rinsing with water or a buffer.
Technical Tip: Principle HPLC Conditions for LC/MS
High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.
Technical Tip: Fronting Peaks
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.
Technical Tip: Choosing & Changing Syringe Wash Solvents
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.
Ghost Peaks Technical Tip: (BOO!) How to Spot Them & Scare Them Away
Peaks in your chromatogram not attributed to the sample injected are commonly referred to as ghost peaks. Several problems can be the cause of ghost peaks manifesting, including: Late Continue Reading
Technical Tip: Optimizing Wash and Elution Protocols in SPE Method Development
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.