There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.
Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.
For most reversed-phase solid phase extraction applications using a silica-based SPE media, cartridges are initially conditioned using an organic solvent such as methanol, followed by rinsing with water or a buffer.
In the first GC columns, gas chromatography phases were simply coated onto the column. This was mostly the case with packed columns, but also the same with the very first capillary columns. Injecting water onto these phases dissolved the phase and caused the phase to elute from the columns.
High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.
