Elution Archives - SCIENCE UNFILTERED

FeaturedSample PreparationTechnical Tips

Solid Phase Extraction FAQs: What is SPE and How Does it Work?

October 11, 2018 Science UnfilteredLeave a comment

Solid Phase Extraction FAQs Answered! Ashmeeta Chera, Phenomenex Sample Preparation Sales Manager and Matt Brusius, Phenomenex Sample Preparation Product Manager, discuss the major Solid Phase Extraction FAQs such as “what is Continue Reading

Tagged 96-well plates,batch processing,catch and release,Concentration,Elution,FAQs,Frequently Asked Questions,liquid liquid extraction,lle,Sample Prep,Sample Preparation,sample preparation products,Solid Phase Extraction,Solid Phase Extraction FAQs,SPE,SPE demonstration,SPE Steps,Strata,traditional SPE

FeaturedSample PreparationTechnical Tips

What is Microelution and What are the Best Strategies When Using It?

July 17, 2018 Science UnfilteredLeave a comment

Microelution Solid Phase Extraction(SPE) is essentially miniaturized SPE. The technique is ideal for analysts that have small or limited sample volumes or for those that want to save time by Continue Reading

Tagged 96 well plate,Analytes,Elution,microelution,microelution spe,Microelution strategies,miniaturized SPE,peptide,peptide screening,Peptide Screening 96-Well Plate,polypropylene,polypropylene collection plates,resoluble,solid phase,Solid Phase Extraction,Sorbent,SPE,SPE format,Strata-X,Strata-X Method Development,thermolabile analytes,well plate

Liquid Chromatography (LC)Technical TipsUncategorized

Best and Worst Organic Solvents to Use in LC

July 14, 2016July 14, 2016 Science Unfiltered1 Comment

There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.

Tagged Acetone,Acetonitrile,Analyte,Analytes,Analytical,Analytical Chemistry,Analytical Science,Backpressure,Backpressures,Best,Chemistry,Chromatogram,Chromatographer,Chromatographers,Chromatography,Column,Column Chromatography,Column Cleaning,Columns,Elute,Elution,Ethanol,Fatty,Flow,Flow Rate,High Backpressure,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,Highly Pigmented,HPLC Column,HPLC System,Iso Propanol,LC,LC/MS/MS,Liquid Chromatography,Liquid Chromatography (LC),Methanol,n-Propanol,Organic,Organic Solvent,Organic Solvents,Peak,Peaks,Pigment,Pigmented,Polarity,Polarity Index,Protein,Proteins,Regeneration,Science,Separation,Separation Sciences,Solubility,Solubilizer,Soluble,Solvent,Solvents,Technical Tips,Tetrahydrofuran,UHPLC,UV,UV Cutoff,UV Detection,UV/Vis,Viscosity,Worst

Gas Chromatography (GC)Technical TipsUncategorized

Technical Tip: Early Eluting Peak Shape

March 1, 2016April 15, 2016 Science UnfilteredLeave a comment

Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.

Tagged Analyte,Analyte Migration,Analytes,Column Film Thickness,Dissolve,Distortion,Distortions,Early,Early Eluting,Early Eluting Peak Shape,Early Eluting Peaks,Early Elution,Eluting,Elution,Film,Film Thickness,Gas Chromatography,Gas Chromatography (GC),GC Column,GC Oven,Glass Wool,Guard,Guard Column,Inject,Injection,Injection Volume,Inlet,Inlet Discrimination,Method,Method Development,Mismatch,Oven,Oven Temperature,Peak,Peak Shape,Peaks,Polarity,Pressure,Pressure Pulsed Injection,Sensitivity,Solvent,Solvent Mismatch,Solvents,Split Injection,Technical Tips,Temperature,Trouble

Sample PreparationTechnical TipsUncategorized

Poor Sample Recovery? You May Be Over-Drying

February 23, 2016April 15, 2016 Science UnfilteredLeave a comment

For most reversed-phase solid phase extraction applications using a silica-based SPE media, cartridges are initially conditioned using an organic solvent such as methanol, followed by rinsing with water or a buffer.

Tagged Analyte,Analyte Loss,Analytes,Buffer,Buffers,C18,Cartridge,Cartridges,Centrifuge,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatography,Conditioning,Drying,Elution,Gravity,Gravity Flow,Loss of Analyte,Methanol,Over-Drying,Polymer,Polymeric,Pressure,QSP,Recovery,Reproducibility,Retention,Separation Sciences,Silica,Silica-Based,Solid Phase Extraction,Sorbent,Sorbents,SPE,Strata,Strata-X,Vacuum,Velocity

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Principle HPLC Conditions for LC/MS

January 18, 2016May 16, 2017 Science UnfilteredLeave a comment

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.