What is HILIC Separation? What Does It Mean For You?

HILIC Separation

By Scott Krepich, Food and Environmental Application Scientist Hydrophilic Interaction Chromatography (HILIC) is a variant of normal-phase liquid chromatography that partly overlaps with other chromatographic applications such as ion-chromatography and Continue Reading

Milton’s Tricks, Treats, and Technical Guides

You’ve worked so hard crafting the perfect costume, decorating your home, and sneaking chocolate from your kids, we decided to treat you to the best technical guides this Halloween.

What the HILIC is HILIC?

Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.

Technical Tip: Peak Apex Flattening

Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.

Technical Tip: Principle HPLC Conditions for LC/MS

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.

Technical Tip: Ion-Pairing Agents

ion exchange sites

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.