HPLC System Archives - SCIENCE UNFILTERED

Liquid Chromatography (LC)Technical TipsUncategorized

Best and Worst Organic Solvents to Use in LC

July 14, 2016July 14, 2016 Science Unfiltered1 Comment

There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.

Tagged Acetone,Acetonitrile,Analyte,Analytes,Analytical,Analytical Chemistry,Analytical Science,Backpressure,Backpressures,Best,Chemistry,Chromatogram,Chromatographer,Chromatographers,Chromatography,Column,Column Chromatography,Column Cleaning,Columns,Elute,Elution,Ethanol,Fatty,Flow,Flow Rate,High Backpressure,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,Highly Pigmented,HPLC Column,HPLC System,Iso Propanol,LC,LC/MS/MS,Liquid Chromatography,Liquid Chromatography (LC),Methanol,n-Propanol,Organic,Organic Solvent,Organic Solvents,Peak,Peaks,Pigment,Pigmented,Polarity,Polarity Index,Protein,Proteins,Regeneration,Science,Separation,Separation Sciences,Solubility,Solubilizer,Soluble,Solvent,Solvents,Technical Tips,Tetrahydrofuran,UHPLC,UV,UV Cutoff,UV Detection,UV/Vis,Viscosity,Worst

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Principle HPLC Conditions for LC/MS

January 18, 2016May 16, 2017 Science UnfilteredLeave a comment

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.

Tagged Absolute Solvent Flow,Acetic,Acetic Acid,Acetonitrile,Acid,Acids,Alkylsulfonates,Analysis,Analyte,Analytes,Analyze,Buffer,Buffers,Challenge,Challenges,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatographic,Chromatography,Column,Columns,Desolvation,Detection,Eluant,Eluent,Elution,Evaporation,Flow,Flow Rate,Formic,Formic Acid,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HILIC,HPLC Column,HPLC System,HPLC Systems,Hydrophilic Interaction Liquid Chromatography,Ion,Ion-Pair Reagent,Ion-Pair Reagents,Ion-Source,Ion-Suppression Zone,Ionizable,LC,LC/MS,LC/MS/MS,Liquid Chromatography,Liquid Chromatography (LC),Mass Spec,Mass Spectral,Mass Spectrometry,Mobile Phase,Non-Volatile,Organic,Organic Acid Buffers,Parameters,Peak,Peak Shape,Peak Shapes,Peaks,pH,Phase,Phases,Phenomenex,Polar,Reversed Phase,Science,Sensitivity,Separation,Separation Sciences,Silica,Solvent,Stationary Phase,System,Systems,TEA,TFA,UHPLC,Void,Volatile,Volatile Acidic Ion-Pair Reagent,Volatile Basic Ion-Pair Reagent,Volatility

Close