by Phenomenex ®
By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration curve, use a standard mobile phase like 1X PBS, and voila, done and done. Of course, that isn’t the case. Continue Reading
When performing reversed phase LC separations, there is a natural tendency of method developers to reach for the most commonly used phase—a C18. However, there are so many different variations of C18 phases available to method developers that it often becomes difficult to choose a C18 column that one had Continue Reading
Meet one of the developers of UHPLC, Jason Anspach, Ph.D.- Research and Development, Senior Scientist here at Phenomenex. Ever read about advancements in science and wonder where and how it all started? When it comes to developments in Ultra High Performance Liquid Chromatography (UHPLC), Jason Anspach doesn’t have to wonder, because he was Continue Reading
Part 1: Changing Column Inner Diameter While Adjusting Flow Rate Guest Author: Dr. Jeff Layne Welcome everyone! Jeff Layne here, back with another article series, and this time we are returning to our method development roots. In this new series, we are going to address the topic of column inner Continue Reading
Guest Author: Jeff Layne Well – it seems that we have a little more work to do with cannabis potency testing. I had originally planned to have the last article be the last of this HPLC method development series, however, we had a request to develop a similar separation using Continue Reading
Selecting the right column for your reversed phase method isn’t always easy. We wanted to help in the decision-making process by breaking down the different steps you’ll need to consider when selecting your column. For a full in depth look into the selection process see our Ultimate Guide to HPLC/UHPLC Continue Reading
(Via LCGC North America) Learn to capitalize on the advantages of both core-shell and thermally modified fully porous stationary phase media to expand the options for HPLC and UHPLC method development. High performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography(UHPLC) method development options can be extended significantly by tapping Continue Reading
Guest author: Dr. Ramkumar Dhandapani Chromatography is coined from the Greek word chroma- which means color and -graphein which means to write. First recorded use of column chromatography can be traced back to Russian scientist Mikhail Tsvet who crushed calcium carbonate into a tube and added homogenized green plant leaves Continue Reading
Guest Author: Brian Rivera, Product Manager: Bio Separations Over a hundred years ago, Nobel Prize laureate, Paul Ehrlich, envisioned a drug which could kill microbes but not harm the body, a “magic bullet” he called it. From mustine to monoclonal antibodies, researchers have implemented Ehrlich’s side chain theory in attempts Continue Reading
Meet the Guru of Liquid Chromatography and Mass Spectrometry…Senior Scientist, Seyed Sadjadi. With 26 plus years in the clinical research industry, Mr. Sadjadi is known company-wide for his insights into Liquid Chromatography and Mass Spectrometry, or LC-MS. From working as a chemist in toxicology, to field applications for LC-MS small Continue Reading