The mobile phase used in HPLC has a profound effect on the separation of the sample by directly affecting the retention and differential migration of the components in the sample. Continue Reading
The Importance of a Well Prepared Mobile Phase Explained
A buffer is an AQUEOUS solution containing a weak acid and its conjugate base (or a weak base and its conjugate acid). The pH of a buffer solution changes very Continue Reading
Derivatizing Reagents for GC – The Buffers Strike Back
Our previous technical tip spoke about silylation reagents that may be used to derivatize a range of polar, acidic, and basic analytes to improve volatility and peak shape during gas chromatography. Silylating Continue Reading
HILIC Technical Tip for Stronger Analytical Methods
Technical Tip: Engineering successful analytical methods using HILIC as an alternative retention mechanism Overview: This technical tip will outline hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) as an alternative retention mechanism that is particularly effective for LC-MS. Continue Reading
Column Temperature – How to Maintain Accuracy and Stability
Guest Author – Genevieve Hodson, Technical Specialist It is common practice to leave LC columns at ‘room temperature’, or ambient temperature conditions when running routine analytical methods. So why do Continue Reading
Inside the Lab with Phenomenex and Industry Expert Panelists
Phenomenex has recently launched a unique web series unlike anything they have done before. “Inside the Lab” invites viewers to not only learn from a panel of experts of the Continue Reading
Will Remdesivir Be the Hope to Help Treat COVID-19?
Guest Author – Grace Guo, Technical Specialist Remdesivir, a nucleoside analog investigation antiviral drug developed by Gilead Sciences, is being studied in several clinical trials as an experimental treatment for Continue Reading
HPLC Column Lifetime – Knowing When It Is Time to Say Goodbye
Guest Author – Dr. Helen Whitby, Technical Specialist Letting go of your favorite column can be hard to do, so when is it really time to say goodbye? Below are Continue Reading
International Beer Day and the Science Behind the Amber Liquid
August 2nd, 2019 marks International Beer Day – a day for beer lovers everywhere to raise a toast to their local brewers and bartenders and appreciate the art of the Continue Reading
Column Care Guide: How to Clean an HPLC Column Using Reverse Flush
Column care is exceptionally important in a lab, however, if not done properly can affect your column’s health. Cleaning your HPLC column might be simple, but it is exceptionally important Continue Reading