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Part 3: What is the Best Column ID for You? Hi, and welcome back to the third installation of this article series on the effect of changing column inner diameter (ID) in chromatography. So far, we have taken the test probe, 17-alpha-OH-progesterone, and run a simple linear gradient on the LC-MS Continue Reading
Did you know that Phenomenex LC columns are a universal fit for all HPLC and UHPLC systems on the market? It’s simple to install Phenomenex UHPLC columns onto Waters® ACQUITY® UPLC® Systems. Here’s how! 1. Disconnect the pre-column heater from the oven. 2. Separated the front and back nuts so Continue Reading
Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.