In the first GC columns, gas chromatography phases were simply coated onto the column. This was mostly the case with packed columns, but also the same with the very first capillary columns. Injecting water onto these phases dissolved the phase and caused the phase to elute from the columns.
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Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.
Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.
Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.