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Liquid Chromatography (LC)Technical Tips

Technical Tip: Principle HPLC Conditions for LC/MS

January 18, 2016May 16, 2017 Science UnfilteredLeave a comment

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.

Tagged Absolute Solvent Flow,Acetic,Acetic Acid,Acetonitrile,Acid,Acids,Alkylsulfonates,Analysis,Analyte,Analytes,Analyze,Buffer,Buffers,Challenge,Challenges,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatographic,Chromatography,Column,Columns,Desolvation,Detection,Eluant,Eluent,Elution,Evaporation,Flow,Flow Rate,Formic,Formic Acid,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HILIC,HPLC Column,HPLC System,HPLC Systems,Hydrophilic Interaction Liquid Chromatography,Ion,Ion-Pair Reagent,Ion-Pair Reagents,Ion-Source,Ion-Suppression Zone,Ionizable,LC,LC/MS,LC/MS/MS,Liquid Chromatography,Liquid Chromatography (LC),Mass Spec,Mass Spectral,Mass Spectrometry,Mobile Phase,Non-Volatile,Organic,Organic Acid Buffers,Parameters,Peak,Peak Shape,Peak Shapes,Peaks,pH,Phase,Phases,Phenomenex,Polar,Reversed Phase,Science,Sensitivity,Separation,Separation Sciences,Silica,Solvent,Stationary Phase,System,Systems,TEA,TFA,UHPLC,Void,Volatile,Volatile Acidic Ion-Pair Reagent,Volatile Basic Ion-Pair Reagent,Volatility

Liquid Chromatography (LC)Technical Tips

Technical Tip: Split Peaks

December 17, 2015April 18, 2016 Science Unfiltered3 Comments

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.

Tagged Application,Applications,Backpressure,Backpressures,Blockage,Buffer,Buffers,Channel,Chromatography,Column,Column Lifetime,Filter,Filtered,Filtering,Flow,Flow Path,Guard Cartridge,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HPLC Column,Injection,Injections,Ionizable,LC/MS/MS,Lifetime,Liquid Chromatography,Liquid Chromatography (LC),Mobile Phase,Peak,Peaks,pH,Phenomenex,pKa,Sample,Samples,Science,Separation Sciences,Split Peak,Split Peaks,Tech Tip,Tech Tips,Technical Tip,Technical Tips,UHPLC,Void

Liquid Chromatography (LC)Technical Tips

Technical Tip: Ion-Pairing Agents

October 18, 2015August 22, 2017 Science UnfilteredLeave a comment

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Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.

Tagged Alkyl,Ammonium,Analyte,Analytes,Chromophore,Column,Counter-ions,Equilibration,Heptane,Hexane,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HILIC,HPLC Column,Hydrocarbon,Hydrocarbon Chain,Hydrophilic Interaction Liquid Chromatography,Hydrophobia,Hydrophobic,Hydrophobicity,Ion,Ion-Pair,Ion-Pair Agent,Ion-Pair Agents,Ion-pair Complex,Ion-Pair Reagent,Ion-Pair Reagents,Ion-Pairing,Ion-Pairing Agents,Ionic,Ionizable,LC,Liquid Chromatography,Liquid Chromatography (LC),Mass Spec,Mass Spectrometry,Octane,Octane-Sulfonic,Octanesulfonic Acid,Peak,Peak Shape,Peaks,Phenylpropanolamine,Polar Analyte,Polar Analytes,Quaternary Ammonium Salts,Retention,Sulfonic,Sulfonic Acid,Technical Tips,Tetrabutylammonium,Tetrabutylammonium Hydroxide,Tetramethyl,Triethylamine,Trifluoroacetic,Trifluoroacetic Acid,UHPLC,UV,UV Detection,Volatile,Volatile Agents,Volatility

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