A Comparison of Various Core-Shell C18 Phases for Method Development

When performing reversed phase LC separations, there is a natural tendency of method developers to reach for the most commonly used phase—a C18. However, there are so many different variations of C18 phases available to method developers that it often becomes difficult to choose a C18 column that one had Continue Reading

The Effects of Changing Column Inner Diameter on LC-MS Applications- Part 2

Part 2: Changing Column Inner Diameter with Constant Flow Rate Guest Author: Dr. Jeff Layne Welcome back to this article series on the effects of changing column inner diameter on LC-MS applications. Well, let’s be fair, we are using LC-MS to illustrate our discussion, but the concepts we cover apply Continue Reading

What is Core-shell and Superficially Porous Technology?

Guest Author: Technical Specialist, Ann Montross Silica media in HPLC columns were originally irregular shaped particles of silica. Then silica based HPLC columns moved to the traditionally filled, fully porous spherical particles of silica that offered improvements in chromatography over irregular shaped particles. Figure 1: Fully Porous Particles As the Continue Reading

Kinetex HPLC/UHPLC Columns Win the Gold Seal of Quality from Leading Industry Publication, SelectScience

    Torrance, Calif. (July, 2017) – Phenomenex Inc, global leader in the research, design, and manufacture of advanced technologies for the separation sciences, announced today that its Kinetex® Core-Shell HPLC/UHPLC columns have won the Gold Seal of Quality by SelectScience, an industry leading online publication. The SelectScience® Gold Seal Continue Reading

Rock Out to Toxicology’s Greatest Hits

This year’s hottest mix is a playlist that will rock on for centuries to come. It has been praised as the album of the year by The Rolling Chromatography* and The New York Toxicology Times* calls it a “massive hit!” Let’s go through the tracks to have a quick listen Continue Reading

Selecting the Right Column for Your Reversed Phase Method

Selecting the right column for your reversed phase method isn’t always easy. We wanted to help in the decision-making process by breaking down the different steps you’ll need to consider when selecting your column. For a full in depth look into the selection process see our Ultimate Guide to HPLC/UHPLC Continue Reading

Webinar Highlights – UHPLC: Small Particle Solutions for Big Chromatography Challenges

Senior Field Application Scientist Scott Krepich answers your questions on UHPLC column selection and shares industry-specific tips In this free webinar, Scott Krepich, Senior Field Application Scientist, Phenomenex Inc., gave an informative presentation on column selection for UHPLC and shared industry-specific tips on method screening and development. Read on for Continue Reading

Jeff Tries Cannabis Part 5: HPLC Method Development

Guest author: Jeff Layne Hello again, and welcome back to my cannabinoid method development article series. From the last post, we were finally able to find a few different columns that could successfully resolve all 12 of our target analytes within the pressure limitations imposed by the customer, and within Continue Reading

Symbiosis of Core-Shell and Fully Porous Particles: Implementing Both for Better HPLC and UHPLC Results

(Via LCGC North America) Learn to capitalize on the advantages of both core-shell and thermally modified fully porous stationary phase media to expand the options for HPLC and UHPLC method development. High performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography(UHPLC) method development options can be extended significantly by tapping Continue Reading

What is HPLC and How Does it Work?

Phenomenex HPLC Columns

Guest author: Dr. Ramkumar Dhandapani Chromatography is coined from the Greek word chroma- which means color and -graphein which means to write. First recorded use of column chromatography can be traced back to Russian scientist Mikhail Tsvet who crushed calcium carbonate into a tube and added homogenized green plant leaves Continue Reading