SPE vs LLE: A Battle of Methods

SPE

An Improved SPE Clean Up and Recovery of Pharmaceutical Compounds from Plasma using Strata® -X  In one corner, we have liquid-liquid extraction (LLE), which is perhaps the most established clean-up Continue Reading

β-Gone β-Glucuronidase Removal FAQs

β-Gone β-Glucuronidase Removal 96-well plates and tubes are designed to target and remove β-glucuronidase from hydrolyzed urine samples without requiring additional time or method development. In a single step and in less than 1 minute, your hydrolyzed samples are ready for analysis.

Best and Worst Organic Solvents to Use in LC

There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.

What the HILIC is HILIC?

Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.

Poor Sample Recovery? You May Be Over-Drying

For most reversed-phase solid phase extraction applications using a silica-based SPE media, cartridges are initially conditioned using an organic solvent such as methanol, followed by rinsing with water or a buffer.

Technical Tip: Optimizing Wash and Elution Protocols in SPE Method Development

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.