Doing HPLC Method Development for Monoclonal Antibody Aggregates? Wait a “SEC”!

By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration curve, use a standard mobile phase like 1X PBS, and voila, done and done. Of course, that isn’t the case. Continue Reading

Can an UHPLC Column Have Too Much Efficiency?

Is 100% aqueous stability and unique polar selectivity not enough to convince you to try Luna Omega? Well, here is another equally-compelling reason Guest Author: Dr. Jeff Layne You Can Never Have Too Much Efficiency! The efficiency of a column is a measurement of the amount of band broadening that Continue Reading

A New Test Uses a Single Drop of Blood to Screen for 13 Types of Cancer

A team of researchers in a Tokyo-based National Cancer Center (NCC) has developed a new blood test that can diagnose 13 types of cancer, as originally reported by The Japan News. With more than 100 types of cancer, there are many variables, and diagnoses can sometimes come too late. The Japanese team Continue Reading

Jeff Tries Cannabis Part 6: HPLC Method Development

Guest Author: Jeff Layne Well – it seems that we have a little more work to do with cannabis potency testing. I had originally planned to have the last article be the last of this HPLC method development series, however, we had a request to develop a similar separation using Continue Reading

Transform Your HPLC and UHPLC Method Development Mindset on Analyzing Proteins— Including Monoclonal Antibodies!

Method development and analyzing proteins

A discussion of wide-pore/core-shell technology for monoclonal antibodies, method development considerations with wide-pore/core-shell columns, size exclusion chromatography (SEC) using sub-2 μm particles, and method optimization for SEC. (Webinar sneak peek) Click Here To View Entire Webinar   Introduction Analyzing Proteins  Analytical chemists have relied on high performance liquid chromatography (HPLC) Continue Reading

What is Chiral?

Chiral chromatography pharmaceutical

What Exactly Does Chiral Mean? Chirality is a special physical property of a molecule which describes its geometry in three-dimensional space. Chiral molecules commonly consist of a carbon atom attached to four different substituents. The substituents can either be atoms or groups of atoms, but each must differ from the Continue Reading

Milton’s Featured Method: Chlorinated Pesticides from Poultry Fat

Concerns surrounding pesticide contamination are not limited to fruits and vegetables. According to Toxics Action Center, pesticides are used almost everywhere: not only in agricultural fields, but also in homes, parks, schools, buildings, forests, and roads.

Technical Tip: Early Eluting Peak Shape

Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.

SPE Method Validation Terms: Precision and Accuracy

In the heart of every good chromatographer is a solid understanding of the most crucial vocabulary. For those practicing solid phase extraction, precision and accuracy are fundamental components to their craft.

Technical Tip: Optimizing Wash and Elution Protocols in SPE Method Development

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.