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By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration curve, use a standard mobile phase like 1X PBS, and voila, done and done. Of course, that isn’t the case. Continue Reading
Is 100% aqueous stability and unique polar selectivity not enough to convince you to try Luna Omega? Well, here is another equally-compelling reason Guest Author: Dr. Jeff Layne You Can Never Have Too Much Efficiency! The efficiency of a column is a measurement of the amount of band broadening that Continue Reading
A team of researchers in a Tokyo-based National Cancer Center (NCC) has developed a new blood test that can diagnose 13 types of cancer, as originally reported by The Japan News. With more than 100 types of cancer, there are many variables, and diagnoses can sometimes come too late. The Japanese team Continue Reading
Guest Author: Jeff Layne Well – it seems that we have a little more work to do with cannabis potency testing. I had originally planned to have the last article be the last of this HPLC method development series, however, we had a request to develop a similar separation using Continue Reading
A discussion of wide-pore/core-shell technology for monoclonal antibodies, method development considerations with wide-pore/core-shell columns, size exclusion chromatography (SEC) using sub-2 μm particles, and method optimization for SEC. (Webinar sneak peek) Click Here To View Entire Webinar Introduction Analyzing Proteins Analytical chemists have relied on high performance liquid chromatography (HPLC) Continue Reading
What Exactly Does Chiral Mean? Chirality is a special physical property of a molecule which describes its geometry in three-dimensional space. Chiral molecules commonly consist of a carbon atom attached to four different substituents. The substituents can either be atoms or groups of atoms, but each must differ from the Continue Reading
Concerns surrounding pesticide contamination are not limited to fruits and vegetables. According to Toxics Action Center, pesticides are used almost everywhere: not only in agricultural fields, but also in homes, parks, schools, buildings, forests, and roads.
Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.
In the heart of every good chromatographer is a solid understanding of the most crucial vocabulary. For those practicing solid phase extraction, precision and accuracy are fundamental components to their craft.
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.