Doing HPLC Method Development for Monoclonal Antibody Aggregates? Wait a “SEC”!

By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration Continue Reading

Can an UHPLC Column Have Too Much Efficiency?

Is 100% aqueous stability and unique polar selectivity not enough to convince you to try Luna Omega? Well, here is another equally-compelling reason Guest Author: Dr. Jeff Layne You Can Continue Reading

Transform Your HPLC and UHPLC Method Development Mindset on Analyzing Proteins— Including Monoclonal Antibodies!

Method development and analyzing proteins

A discussion of wide-pore/core-shell technology for monoclonal antibodies, method development considerations with wide-pore/core-shell columns, size exclusion chromatography (SEC) using sub-2 μm particles, and method optimization for SEC. (Webinar sneak peek) Continue Reading

Milton’s Featured Method: Chlorinated Pesticides from Poultry Fat

Concerns surrounding pesticide contamination are not limited to fruits and vegetables. According to Toxics Action Center, pesticides are used almost everywhere: not only in agricultural fields, but also in homes, parks, schools, buildings, forests, and roads.

Technical Tip: Early Eluting Peak Shape

Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.

SPE Method Validation Terms: Precision and Accuracy

In the heart of every good chromatographer is a solid understanding of the most crucial vocabulary. For those practicing solid phase extraction, precision and accuracy are fundamental components to their craft.