The mobile phase used in HPLC has a profound effect on the separation of the sample by directly affecting the retention and differential migration of the components in the sample. Continue Reading
Guest Author – Helen Whitby, PhD A buffer is an AQUEOUS solution containing a weak acid and its conjugate base (or a weak base and its conjugate acid). The pH of a buffer solution changes Continue Reading
Guest Author – Helen Whitby, PhD, Technical Specialist Micro LC columns offer chromatographers the opportunity to improve sensitivity or use lower flow rates due to their smaller internal diameter. The Continue Reading
Guest Author – James Turner, Technical Specialist Manager Basic compounds have long been a challenge for chromatographers. Reversed phase separation offers a flexible and effective way to separate compounds based Continue Reading
Guest Author – Technical Expert Genevieve Hodson Liquid Chromatography is reliant upon a accurately made mobile phase. Many chromatographic problems can be attributed to an incorrectly prepared mobile phase. Contamination Continue Reading
Guest Author: Grace Guo, Phenomenex Technical Specialist Ion-exclusion columns, such as the Phenomenex Rezex column, have been widely used on the analysis of sugars and organic acids. The aqueous based Continue Reading
Preparative chromatography is a specialized subset of chromatography, where the goal is to purify a target analyte (or analytes in some cases) from a complex mixture of crude sample. The Continue Reading
Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.