Peak Shape Archives - SCIENCE UNFILTERED

Liquid Chromatography (LC)Technical TipsUncategorized

10 Questions You’ve Always Wanted to Ask About LC

June 14, 2016August 22, 2017 Science Unfiltered3 Comments

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Tagged Analytes,Analytical Chemistry,Analytical Science,Chem,Chemical,Chemical Disposal,Chemicals,Chemistry,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatography,Column,Column Chromatography,Column Lifetime,Columns,Connective Fitting,Connective Fittings,Damage,Dewetting,End-Fitting,End-Fittings,Equilibration,Gradient,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HPLC Column,Inlet,Inlet Threading,Ion-Pair,Ion-Pair Agent,Ion-Pair Agents,Ion-Pair Reagent,Ion-Pair Reagents,Ion-Pairing Agents,Ion-Pairing Reagents,LC,LC Column,LC/MS/MS,Leak,Leaking,Leaky Column,Lifetime,Liquid Chromatography (LC),MSDS,Organic Solvent,Peak,Peak Broadening,Peak Shape,Peaks,Phase,Phase Collapse,Phases,Phenomenex,Pressure,Re-Equilibration,Recycling Organisation for Research Opportunities,Resolution,Retention,RORO,Science,Separation,Separation Sciences,Silica,Solvent,Solvents,Split Peaks,Splitting,Technical Tips,UHPLC,Wetting

Gas Chromatography (GC)Technical TipsUncategorized

Technical Tip: Early Eluting Peak Shape

March 1, 2016April 15, 2016 Science UnfilteredLeave a comment

Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.

Tagged Analyte,Analyte Migration,Analytes,Column Film Thickness,Dissolve,Distortion,Distortions,Early,Early Eluting,Early Eluting Peak Shape,Early Eluting Peaks,Early Elution,Eluting,Elution,Film,Film Thickness,Gas Chromatography,Gas Chromatography (GC),GC Column,GC Oven,Glass Wool,Guard,Guard Column,Inject,Injection,Injection Volume,Inlet,Inlet Discrimination,Method,Method Development,Mismatch,Oven,Oven Temperature,Peak,Peak Shape,Peaks,Polarity,Pressure,Pressure Pulsed Injection,Sensitivity,Solvent,Solvent Mismatch,Solvents,Split Injection,Technical Tips,Temperature,Trouble

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Peak Apex Flattening

February 19, 2016April 15, 2016 Science UnfilteredLeave a comment

Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.

Tagged Absorbance,Analysis,Analytical,Analytical Chemistry,Analytical Science,Apex,Assay,Beer's Law,Beer-Lambert,Beer-Lambert Equation,Beer-Lambert Law,Calibration,Chemistry,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatography,Co-Efficient,Column,Column Chromatography,Columns,Concentration,Core-Shell,Detection,Detector,Detector Saturation,Extinction,Flat,Flat Peak,Flat Peaks,Flattening,Fraction,Fraction Collecting,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,High-Load:Low-Load,HILIC,HPLC Column,HPLC/UHPLC,Impurities,Impurity,Inject,Injection,Ion Exchange,Ion Exclusion,Lambert-Beer Law,Linear Calibration,Liquid Chromatography (LC),Molecule,Molecules,Noise,Normal Phase,Path Lengths,Peak,Peak Apex,Peak Apex Flattening,Peak Flattening,Peak Height,Peak Shape,Peaks,Quantification,Quantify,Reversed Phase,Sample,Samples,Saturation,Science,Separation Sciences,Transmittance,UHPLC,UHPLC Column,UV,UV Detection,UV/Vis

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Principle HPLC Conditions for LC/MS

January 18, 2016May 16, 2017 Science UnfilteredLeave a comment

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.