Tag: <span>Peaks</span>

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Chromatographic Peaks and Syringe Filtration

April 29, 2021April 14, 2021 Science UnfilteredLeave a comment

chromatographic peaks and syringe filters

I have an intermittent issue with chromatographic peaks appearing in my analysis. I think they might be coming from my syringe filters, what should I do? It is possible that Continue Reading

Tagged chromatographic peaks,Peaks,Phenex syringe filters,Syringe,syringe filters,syringe filtration

Liquid Chromatography (LC)Technical TipsUncategorized

Best and Worst Organic Solvents to Use in LC

July 14, 2016July 14, 2016 Science Unfiltered1 Comment

There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.

Tagged Acetone,Acetonitrile,Analyte,Analytes,Analytical,Analytical Chemistry,Analytical Science,Backpressure,Backpressures,Best,Chemistry,Chromatogram,Chromatographer,Chromatographers,Chromatography,Column,Column Chromatography,Column Cleaning,Columns,Elute,Elution,Ethanol,Fatty,Flow,Flow Rate,High Backpressure,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,Highly Pigmented,HPLC Column,HPLC System,Iso Propanol,LC,LC/MS/MS,Liquid Chromatography,Liquid Chromatography (LC),Methanol,n-Propanol,Organic,Organic Solvent,Organic Solvents,Peak,Peaks,Pigment,Pigmented,Polarity,Polarity Index,Protein,Proteins,Regeneration,Science,Separation,Separation Sciences,Solubility,Solubilizer,Soluble,Solvent,Solvents,Technical Tips,Tetrahydrofuran,UHPLC,UV,UV Cutoff,UV Detection,UV/Vis,Viscosity,Worst

Liquid Chromatography (LC)Technical TipsUncategorized

What the HILIC is HILIC?

June 23, 2016December 9, 2016 Science Unfiltered1 Comment

Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.

Tagged Acetate,Acetonitrile,Amino,Ammonium,Ammonium Acetate,Ammonium Formate,Analysis,Analyte,Analytes,Analytical,Analytical Chemistry,Analytical Science,Aqueous,Ascorbic acid,Bilayer,Buffer,Buffers,Chemistry,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatography,CMP,Column,Column Chromatography,Columns,Cytidine Monophosphate,Detection,Diol,Diol Bonded,Folic Acid,Formate,GMP,Guanosine Monophosphate,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HILIC,HPLC Column,Hydrophilic,Hydrophilic Interaction Liquid Chromatography,IMP,Injection,Injections,Inosine Monophosphate,LC,LC/MS/MS,Liquid,Liquid Chromatography,Liquid Chromatography (LC),Luna,Methanol,Mobile Phase,Monophosphate,Nicotinamide,Nicotinic acid,Organic,Organic Solvent,PABA,Peak,Peaks,Pentafluorophenyl,pH,Phenomenex,Pyridoxine,Reversed Phase,Riboflavin,Science,Sensitivity,Separation,Separation Sciences,Solvent,Solvents,Stationary Phase,Technical Tips,Thiamine,Thymidine Monophosphate,TMP,UHPLC,UMP,Unbonded Silica,Uridine Monophosphate,Vitamin B12,Water

Liquid Chromatography (LC)Technical TipsUncategorized

10 Questions You’ve Always Wanted to Ask About LC

June 14, 2016August 22, 2017 Science Unfiltered3 Comments

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Tagged Analytes,Analytical Chemistry,Analytical Science,Chem,Chemical,Chemical Disposal,Chemicals,Chemistry,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatography,Column,Column Chromatography,Column Lifetime,Columns,Connective Fitting,Connective Fittings,Damage,Dewetting,End-Fitting,End-Fittings,Equilibration,Gradient,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HPLC Column,Inlet,Inlet Threading,Ion-Pair,Ion-Pair Agent,Ion-Pair Agents,Ion-Pair Reagent,Ion-Pair Reagents,Ion-Pairing Agents,Ion-Pairing Reagents,LC,LC Column,LC/MS/MS,Leak,Leaking,Leaky Column,Lifetime,Liquid Chromatography (LC),MSDS,Organic Solvent,Peak,Peak Broadening,Peak Shape,Peaks,Phase,Phase Collapse,Phases,Phenomenex,Pressure,Re-Equilibration,Recycling Organisation for Research Opportunities,Resolution,Retention,RORO,Science,Separation,Separation Sciences,Silica,Solvent,Solvents,Split Peaks,Splitting,Technical Tips,UHPLC,Wetting

Humanity in ScienceLiquid Chromatography (LC)Our CompanyScience NewsUncategorized

Humanity in Science: Combating Counterfeit with Capillary Electrophoresis

April 26, 2016September 1, 2016 Science Unfiltered1 Comment

The Humanity in Science Award was created in 2015 to recognize outstanding developments in science that could change human lives for the better. There were many runners-up who did (and do!) amazing things for humanity. Recently, The Analytical Scientist sat down with each of them to catch up, and find out how their ventures have progressed in the last 12 months.

Tagged Africa,Analytical,Analytical Chemistry,Analytical Science,Antimalarial,Antimalarial Medication,Atom,Atoms,Award,Capacity,Capillary,Capillary Electrophoresis,CDC,CE,Centers for Disease Control and Prevention,Chromatography,Column,Columns,Cost,Counterfeit,Counterfeited,Detect,Detection,Developing Countries,Drug,Drugs,Electric,Electric Field,Electrokinetic,Electrokinetic Separation,Electrokinetic Separation Techniques,Electrophoresis,Electrophoretic,Electrophoretic Mobility,Emerging Countries,Evaluation,Fake,Feature,Flat Flow,Flow,Flow Rate,Fraud,Fraudulent,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,Humanity in Science,Humanity in Science Award,Julie Schappler,Liquid Chromatography (LC),Malaria,Malarial,Medication,Medicine,Medicine Evaluation,Meds,Mobility,Parabolic Flow,Peak,Peak Capacity,Peaks,Pharma,Pharmaceutical,Pharmaceuticals,Pharmacology,Phenomenex,Philanthropy,Science,Separation Sciences,Serge Rudaz,Smartphone,Solvent,Spotlight,The Analytical Scientist,The Humanity in Science Award,UC Davis,UHPLC,WHO,World Health Organization

Gas Chromatography (GC)Technical TipsUncategorized

Technical Tip: Early Eluting Peak Shape

March 1, 2016April 15, 2016 Science UnfilteredLeave a comment

Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.

Tagged Analyte,Analyte Migration,Analytes,Column Film Thickness,Dissolve,Distortion,Distortions,Early,Early Eluting,Early Eluting Peak Shape,Early Eluting Peaks,Early Elution,Eluting,Elution,Film,Film Thickness,Gas Chromatography,Gas Chromatography (GC),GC Column,GC Oven,Glass Wool,Guard,Guard Column,Inject,Injection,Injection Volume,Inlet,Inlet Discrimination,Method,Method Development,Mismatch,Oven,Oven Temperature,Peak,Peak Shape,Peaks,Polarity,Pressure,Pressure Pulsed Injection,Sensitivity,Solvent,Solvent Mismatch,Solvents,Split Injection,Technical Tips,Temperature,Trouble

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Peak Apex Flattening

February 19, 2016April 15, 2016 Science UnfilteredLeave a comment

Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.

Tagged Absorbance,Analysis,Analytical,Analytical Chemistry,Analytical Science,Apex,Assay,Beer's Law,Beer-Lambert,Beer-Lambert Equation,Beer-Lambert Law,Calibration,Chemistry,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatography,Co-Efficient,Column,Column Chromatography,Columns,Concentration,Core-Shell,Detection,Detector,Detector Saturation,Extinction,Flat,Flat Peak,Flat Peaks,Flattening,Fraction,Fraction Collecting,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,High-Load:Low-Load,HILIC,HPLC Column,HPLC/UHPLC,Impurities,Impurity,Inject,Injection,Ion Exchange,Ion Exclusion,Lambert-Beer Law,Linear Calibration,Liquid Chromatography (LC),Molecule,Molecules,Noise,Normal Phase,Path Lengths,Peak,Peak Apex,Peak Apex Flattening,Peak Flattening,Peak Height,Peak Shape,Peaks,Quantification,Quantify,Reversed Phase,Sample,Samples,Saturation,Science,Separation Sciences,Transmittance,UHPLC,UHPLC Column,UV,UV Detection,UV/Vis

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Principle HPLC Conditions for LC/MS

January 18, 2016May 16, 2017 Science UnfilteredLeave a comment

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.