10 Questions You’ve Always Wanted to Ask About LC

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How Does Temperature Affect a Compound’s Retention Time?

compound retention

The boiling point is the temperature where liquid has enough energy that molecules transferring into the vapor phase exert a pressure that is equal to the outside pressure. This allows molecules anywhere—not just the surface—to transfer to the gas phase.

Technical Tip: Principle HPLC Conditions for LC/MS

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.