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Liquid Chromatography (LC)Technical TipsUncategorized

10 Questions You’ve Always Wanted to Ask About LC

June 14, 2016August 22, 2017 Science Unfiltered3 Comments

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Tagged Analytes,Analytical Chemistry,Analytical Science,Chem,Chemical,Chemical Disposal,Chemicals,Chemistry,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatography,Column,Column Chromatography,Column Lifetime,Columns,Connective Fitting,Connective Fittings,Damage,Dewetting,End-Fitting,End-Fittings,Equilibration,Gradient,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HPLC Column,Inlet,Inlet Threading,Ion-Pair,Ion-Pair Agent,Ion-Pair Agents,Ion-Pair Reagent,Ion-Pair Reagents,Ion-Pairing Agents,Ion-Pairing Reagents,LC,LC Column,LC/MS/MS,Leak,Leaking,Leaky Column,Lifetime,Liquid Chromatography (LC),MSDS,Organic Solvent,Peak,Peak Broadening,Peak Shape,Peaks,Phase,Phase Collapse,Phases,Phenomenex,Pressure,Re-Equilibration,Recycling Organisation for Research Opportunities,Resolution,Retention,RORO,Science,Separation,Separation Sciences,Silica,Solvent,Solvents,Split Peaks,Splitting,Technical Tips,UHPLC,Wetting

Sample PreparationTechnical TipsUncategorized

Poor Sample Recovery? You May Be Over-Drying

February 23, 2016April 15, 2016 Science UnfilteredLeave a comment

For most reversed-phase solid phase extraction applications using a silica-based SPE media, cartridges are initially conditioned using an organic solvent such as methanol, followed by rinsing with water or a buffer.

Tagged Analyte,Analyte Loss,Analytes,Buffer,Buffers,C18,Cartridge,Cartridges,Centrifuge,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatography,Conditioning,Drying,Elution,Gravity,Gravity Flow,Loss of Analyte,Methanol,Over-Drying,Polymer,Polymeric,Pressure,QSP,Recovery,Reproducibility,Retention,Separation Sciences,Silica,Silica-Based,Solid Phase Extraction,Sorbent,Sorbents,SPE,Strata,Strata-X,Vacuum,Velocity

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Fronting Peaks

December 10, 2015April 18, 2016 Science UnfilteredLeave a comment

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.

Tagged Chromatogram,Chromatography,Column,Column Chromatography,Columns,Connective Fittings,Diluent,Elution,Fingertight,Fittings,Flushing,Fronting,Fronting Peaks,HPLC Column,Immiscible,Inject,Injection,Injections,Installation,Iso-Octane,Liquid Chromatography (LC),Mobile Phase,Needle Wash,Overload,Overloading,Peak,Peak Shape,Peaks,PEEK,pH,pHs,Port Depth,Retention,Reversed Phase,Separation Sciences,Solvent,Solvents,Swage,Swaged,UHPLC

Liquid Chromatography (LC)Technical Tips

Technical Tip: Ion-Pairing Agents

October 18, 2015August 22, 2017 Science UnfilteredLeave a comment

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.

Tagged Alkyl,Ammonium,Analyte,Analytes,Chromophore,Column,Counter-ions,Equilibration,Heptane,Hexane,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HILIC,HPLC Column,Hydrocarbon,Hydrocarbon Chain,Hydrophilic Interaction Liquid Chromatography,Hydrophobia,Hydrophobic,Hydrophobicity,Ion,Ion-Pair,Ion-Pair Agent,Ion-Pair Agents,Ion-pair Complex,Ion-Pair Reagent,Ion-Pair Reagents,Ion-Pairing,Ion-Pairing Agents,Ionic,Ionizable,LC,Liquid Chromatography,Liquid Chromatography (LC),Mass Spec,Mass Spectrometry,Octane,Octane-Sulfonic,Octanesulfonic Acid,Peak,Peak Shape,Peaks,Phenylpropanolamine,Polar Analyte,Polar Analytes,Quaternary Ammonium Salts,Retention,Sulfonic,Sulfonic Acid,Technical Tips,Tetrabutylammonium,Tetrabutylammonium Hydroxide,Tetramethyl,Triethylamine,Trifluoroacetic,Trifluoroacetic Acid,UHPLC,UV,UV Detection,Volatile,Volatile Agents,Volatility

Technical Tips

Technical Tip: (BOO!) Ghost Peaks

October 8, 2015April 21, 2016 Science UnfilteredLeave a comment

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.

Tagged Ask a Phenom,Buffers,Carryover,Chromatogram,Chromatography,City Three Peaks,Contamination,Elution,Equilibration,Ghost Peaks,Impurities,Injections,Injector Valve,Late Eluting Peaks,Liquid Chromatography (LC),Mobile Phase,Needle Wash,Purification,Retention,Rotor Seal,Samples,Separation Sciences,Solvent Impurities,Solvents,Technical Support,Technical Tips,Troubleshooting,UHPLC

Tag: Retention

10 Questions You’ve Always Wanted to Ask About LC

Poor Sample Recovery? You May Be Over-Drying

Technical Tip: Fronting Peaks

Technical Tip: Ion-Pairing Agents

Technical Tip: (BOO!) Ghost Peaks

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