Reversed Phase Archives - SCIENCE UNFILTERED

FeaturedLiquid Chromatography (LC)Pharma / BiopharmaTechnical Tips

What is Drug Antibody Ratio and How Does It Relate to Biotherapeutics?

June 5, 2018 Science UnfilteredLeave a comment

Meet James Song, R&D Research Scientist– Bioseparations James has over 20 years of experience in the arena of biotechnology and has a wealth of experience in the fields of diagnostic Continue Reading

Tagged adc,ADC characterization,antibody drug conjugates,biodistribution,biopharm,biopharmaceuticals,bioseparations,biotherapeutics,Chemistry,DAR,drug antibody ratio,drug conjugated,HIC,HPLC,HPLC Methods,HPLC techniques,hydrophobic interaction chromatography,lc-ms,LC-MS/MS,lysine,pharmacokinetics,research scientist,Reversed Phase,UV-Vis,UV-Vis spectroscopy

FeaturedLiquid Chromatography (LC)Pharma / BiopharmaTechnical Tips

Large vs Small Organic Molecules in Biopharmaceuticals

April 17, 2018April 17, 2018 Science UnfilteredLeave a comment

Biopharmaceuticals have emerged as the next-gen of therapeutic (large and small) molecules in the pharmaceutical industry Guest Author: Chad Eichman, Ph.D., Biopharmaceutical Industry Manager The pharmaceutical industry has a long-standing Continue Reading

Tagged adalimumab,Aspirin,big molecules,biologics,biologics analysis,biopharm,biopharmaceuticals,bioZen,chemical syntheses,Chromatography,Dalton,HIC,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HILIC,HILIC Separation,Humira,hydrophilic interaction chromatography,hydrophobic interaction chromatography,IEX,ion exchange chromatography,large molecules,Liquid Chromatography,Liquid Chromatography (LC),Mass Spectrometry,paclitaxel,Pharma,Pharmaceutical,pharmaceutical industry,Pharmaceuticals,post-translational modifications,PTMs,Reversed Phase,RP,sec,size exclusion chromatography,small molecules,small organic molecule,The United States Pharmacopoeia,zen,ZenMaster

FeaturedLiquid Chromatography (LC)Technical Tips

What is HILIC Separation? What Does It Mean For You?

February 15, 2018June 20, 2018 Science UnfilteredLeave a comment

La cromatografía de interacción hidrofílica

By Scott Krepich, Food and Environmental Application Scientist Hydrophilic Interaction Chromatography (HILIC) is a variant of normal-phase liquid chromatography that partly overlaps with other chromatographic applications such as ion-chromatography and Continue Reading

Tagged Acetonitrile,Bioanalytical,HILIC,HILIC elution,HILIC Separation,Hydrophilic,Hydrophilic Interaction Liquid Chromatography,Ion Exchange,LC-MS/MS,Normal Phase,Polar Analytes,Reversed Phase

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Seleccione la Columna Correcta Para su Método de Fase Reversa

January 8, 2018February 20, 2018 Science UnfilteredLeave a comment

No siempre es tarea fácil seleccionar la columna correcta para su método de fase reversa. Nuestro propósito aquí es ayudarle en el proceso de toma de decisiones mediante un desglose Continue Reading

Tagged Espanol,HPLC,Método de Fase Reversa,Reversed Phase,UHPLC

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¿Qué es la HPLC y Cómo Funciona?

December 18, 2017February 20, 2018 Science UnfilteredLeave a comment

Autor invitado: Dr. Ramkumar Dhandapani El término cromatografía procede de la palabra griega chroma- que significa color y -graphein que significa escribir. El primer uso registrado de la cromatografía en Continue Reading

Tagged HPLC Methods,polar interactions,Reversed Phase,UHPLC

FeaturedLiquid Chromatography (LC)Seyed Sadjadi- Senior ScientistTechnical TipsThe Column Doctor Is In

Why is C18 the Most Popular Stationary Phase for Reversed-Phase?

October 5, 2017February 6, 2018 Science UnfilteredLeave a comment

Guest Author: Seyed Sadjadi, Senior Scientist We are going to explore the reasons why C18 stationary phase is the most used when it comes to reversed-phase and how it Continue Reading

Tagged C18,Hydrophobic Stationary phase,Reversed Phase,RPC Retention Mechanism,Stationary Phase

FeaturedLiquid Chromatography (LC)Pharma / BiopharmaScience NewsSitting With Scientists

Peptide Synthesis Technologies with Matteo Villian, Ph.D.

September 28, 2017October 4, 2017 Science UnfilteredLeave a comment

Interview with Peptide Synthesis expert, Matteo Villain, Vice President of Research and Development at Bachem Americas This is a look into the Peptide Purification Industry from the eyes of Matteo Continue Reading

Tagged chromatographic purifications,HPLC,peptide synthesis,PhenoPrep,Purify,Reversed Phase

FeaturedJeff Layne, Ph.D.- LC Product Manager and Technical SpecialistLiquid Chromatography (LC)Technical Tips

Selectivity for Polar Acids

August 2, 2017September 22, 2017 Science Unfiltered1 Comment

Guest Author: Dr. Jeff Layne Is super-high efficiency and 100 % aqueous stability not enough to convince you to try Luna® Omega UHPLC Columns? Well then, how about a unique Continue Reading

Tagged LC,luna omega,Polar Acid,Reversed Phase,Selectivity,UHPLC

FeaturedLiquid Chromatography (LC)Seyed Sadjadi- Senior ScientistTechnical TipsThe Column Doctor Is In

The Stages of Column Death!

July 11, 2017August 14, 2017 Science UnfilteredLeave a comment

Guest Author: Senior Scientist, Seyed Sadjadi. Congratulations! You are the proud new owner of a small stainless-steel tube packed with white sand, which will bring you fabulous results…if it lasts Continue Reading

Tagged Column Chromatography,Column Lifetime,Guard Column,Liquid Chromatography,Particles,Reversed Phase

FeaturedLiquid Chromatography (LC)Pharma / Biopharma

Selecting the Right Column for Your Reversed Phase Method

June 22, 2017January 15, 2018 Science UnfilteredLeave a comment

Selecting the right column for your reversed phase method isn’t always easy. We wanted to help in the decision-making process by breaking down the different steps you’ll need to consider Continue Reading

Tagged Column Selection,Guide,HPLC,Kinetex,luna omega,Reversed Phase,Solid Support,UHPLC

Tag: Reversed Phase