Tag: <span>Reversed Phase</span>

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¿Qué es la HPLC y Cómo Funciona?

June 14, 2022June 27, 2022 Science UnfilteredLeave a comment

Autor invitado: Dr. Ramkumar Dhandapani El término cromatografía procede de la palabra griega chroma- que significa color y -graphein que significa escribir. El primer uso registrado de la cromatografía en Continue Reading

Tagged HPLC Methods,polar interactions,Reversed Phase,UHPLC

FeaturedLiquid Chromatography (LC)

Synthetic Oligonucleotides – Analysis of Their Complex Structure

June 23, 2020June 23, 2020 Science UnfilteredLeave a comment

Guest Author – Helen Whitby, PhD., Technical Specialist Synthetic oligonucleotides are promising therapeutic agents and their characterization is fundamental to their success. However, various impurities either product-related or arising from Continue Reading

Tagged Antisense Oligonucleotides,C18,C18 selectivities,Oligonucleotides,Reversed Phase,RNA Oligonucleotides,Synthetic oligonucleotides

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Analysis of Bases – The Chromatography Method of the Old and New

April 14, 2020April 14, 2020 Science UnfilteredLeave a comment

Guest Author – James Turner, Technical Specialist Manager Basic compounds have long been a challenge for chromatographers. Reversed phase separation offers a flexible and effective way to separate compounds based Continue Reading

Tagged bases,C18 Reagents,Compounds,endcapped,LC-MS/MS,Mobile Phase,pH Stability,Reversed Phase

FeaturedLiquid Chromatography (LC)Pharma / BiopharmaTechnical Tips

What is Drug Antibody Ratio and How Does It Relate to Biotherapeutics?

June 5, 2018 Science UnfilteredLeave a comment

Meet James Song, R&D Research Scientist– Bioseparations James has over 20 years of experience in the arena of biotechnology and has a wealth of experience in the fields of diagnostic Continue Reading

Tagged adc,ADC characterization,antibody drug conjugates,biodistribution,biopharm,biopharmaceuticals,bioseparations,biotherapeutics,Chemistry,DAR,drug antibody ratio,drug conjugated,HIC,HPLC,HPLC Methods,HPLC techniques,hydrophobic interaction chromatography,lc-ms,LC-MS/MS,lysine,pharmacokinetics,research scientist,Reversed Phase,UV-Vis,UV-Vis spectroscopy

FeaturedLiquid Chromatography (LC)Pharma / BiopharmaTechnical Tips

Large vs Small Organic Molecules in Biopharmaceuticals

April 17, 2018April 17, 2018 Science UnfilteredLeave a comment

Biopharmaceuticals have emerged as the next-gen of therapeutic (large and small) molecules in the pharmaceutical industry Guest Author: Chad Eichman, Ph.D., Biopharmaceutical Industry Manager The pharmaceutical industry has a long-standing Continue Reading

Tagged adalimumab,Aspirin,big molecules,biologics,biologics analysis,biopharm,biopharmaceuticals,bioZen,chemical syntheses,Chromatography,Dalton,HIC,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HILIC,HILIC Separation,Humira,hydrophilic interaction chromatography,hydrophobic interaction chromatography,IEX,ion exchange chromatography,large molecules,Liquid Chromatography,Liquid Chromatography (LC),Mass Spectrometry,paclitaxel,Pharma,Pharmaceutical,pharmaceutical industry,Pharmaceuticals,post-translational modifications,PTMs,Reversed Phase,RP,sec,size exclusion chromatography,small molecules,small organic molecule,The United States Pharmacopoeia,zen,ZenMaster

FeaturedLiquid Chromatography (LC)Technical Tips

What is HILIC Separation? What Does It Mean For You?

February 15, 2018June 20, 2018 Science UnfilteredLeave a comment

La cromatografía de interacción hidrofílica

By Scott Krepich, Food and Environmental Application Scientist Hydrophilic Interaction Chromatography (HILIC) is a variant of normal-phase liquid chromatography that partly overlaps with other chromatographic applications such as ion-chromatography and Continue Reading

Tagged Acetonitrile,Bioanalytical,HILIC,HILIC elution,HILIC Separation,Hydrophilic,Hydrophilic Interaction Liquid Chromatography,Ion Exchange,LC-MS/MS,Normal Phase,Polar Analytes,Reversed Phase

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Seleccione la Columna Correcta Para su Método de Fase Reversa

January 8, 2018February 20, 2018 Science UnfilteredLeave a comment

[sg_popup id=”108″ event=”onload”][/sg_popup]No siempre es tarea fácil seleccionar la columna correcta para su método de fase reversa. Nuestro propósito aquí es ayudarle en el proceso de toma de decisiones mediante Continue Reading

Tagged Espanol,HPLC,Método de Fase Reversa,Reversed Phase,UHPLC

FeaturedLiquid Chromatography (LC)Technical TipsThe Column Doctor Is In

Why is C18 the Most Popular Stationary Phase for Reversed-Phase?

October 5, 2017October 9, 2019 Science Unfiltered1 Comment

Guest Author: Seyed Sadjadi, Senior Scientist We are going to explore the reasons why C18 stationary phase is the most used when it comes to reversed-phase and how it works. Continue Reading

Tagged C18,Hydrophobic Stationary phase,Reversed Phase,RPC Retention Mechanism,Stationary Phase

FeaturedLiquid Chromatography (LC)Pharma / BiopharmaScience NewsSitting With Scientists

Peptide Synthesis Technologies with Matteo Villian, Ph.D.

September 28, 2017October 4, 2017 Science UnfilteredLeave a comment

Interview with Peptide Synthesis expert, Matteo Villain, Vice President of Research and Development at Bachem Americas This is a look into the Peptide Purification Industry from the eyes of Matteo Continue Reading

Tagged chromatographic purifications,HPLC,peptide synthesis,PhenoPrep,Purify,Reversed Phase

FeaturedLiquid Chromatography (LC)Technical Tips

Selectivity for Polar Acids

August 2, 2017September 22, 2017 Science Unfiltered1 Comment

Guest Author: Dr. Jeff Layne Is super-high efficiency and 100 % aqueous stability not enough to convince you to try Luna® Omega UHPLC Columns? Well then, how about a unique Continue Reading

Tagged LC,luna omega,Polar Acid,Reversed Phase,Selectivity,UHPLC

Tag: Reversed Phase