Selectivity for Polar Acids

Guest Author: Dr. Jeff Layne Is super-high efficiency and 100 % aqueous stability not enough to convince you to try Luna® Omega UHPLC Columns? Well then, how about a unique selectivity for polar acidic molecules? Selectivity for Polar Acids is Sweet! Developing robust methods for the analysis of small, polar Continue Reading

The Stages of Column Death!

Guest Author: Senior Scientist, Seyed Sadjadi. Congratulations! You are the proud new owner of a small stainless-steel tube packed with white sand, which will bring you fabulous results…if it lasts its life expectancy. This is the first in a series of articles to bring to light the life and death Continue Reading

Selecting the Right Column for Your Reversed Phase Method

Selecting the right column for your reversed phase method isn’t always easy. We wanted to help in the decision-making process by breaking down the different steps you’ll need to consider when selecting your column. For a full in depth look into the selection process see our Ultimate Guide to HPLC/UHPLC Continue Reading

What is HPLC and How Does it Work?

Phenomenex HPLC Columns

Guest author: Dr. Ramkumar Dhandapani Chromatography is coined from the Greek word chroma- which means color and -graphein which means to write. First recorded use of column chromatography can be traced back to Russian scientist Mikhail Tsvet who crushed calcium carbonate into a tube and added homogenized green plant leaves Continue Reading

What the HILIC is HILIC?

Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.

Technical Tip: Peak Apex Flattening

Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.

Technical Tip: Principle HPLC Conditions for LC/MS

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.

Technical Tip: Fronting Peaks

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.