Why is C18 the Most Popular Stationary Phase for Reversed-Phase?

C18 Stationary Phase

Guest Author: Seyed Sadjadi, Senior Scientist   We are going to explore the reasons why C18 stationary phase is the most used when it comes to reversed-phase and how it Continue Reading

Peptide Synthesis Technologies with Matteo Villian, Ph.D.

Peptide Synthesis

Interview with Peptide Synthesis expert, Matteo Villain, Vice President of Research and Development at Bachem Americas This is a look into the Peptide Purification Industry from the eyes of Matteo Continue Reading

The Stages of Column Death!

Guest Author: Senior Scientist, Seyed Sadjadi. Congratulations! You are the proud new owner of a small stainless-steel tube packed with white sand, which will bring you fabulous results…if it lasts Continue Reading

What the HILIC is HILIC?

Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.

Technical Tip: Peak Apex Flattening

Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.

Technical Tip: Principle HPLC Conditions for LC/MS

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.

Technical Tip: Fronting Peaks

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.