Guest Author: Seyed Sadjadi, Senior Scientist We are going to explore the reasons why C18 stationary phase is the most used when it comes to reversed-phase and how it Continue Reading
Peptide Synthesis Technologies with Matteo Villian, Ph.D.
Interview with Peptide Synthesis expert, Matteo Villain, Vice President of Research and Development at Bachem Americas This is a look into the Peptide Purification Industry from the eyes of Matteo Continue Reading
FeaturedJeff Layne, Ph.D.- LC Product Manager and Technical SpecialistLiquid Chromatography (LC)Technical Tips
Selectivity for Polar Acids
Guest Author: Dr. Jeff Layne Is super-high efficiency and 100 % aqueous stability not enough to convince you to try Luna® Omega UHPLC Columns? Well then, how about a unique Continue Reading
FeaturedLiquid Chromatography (LC)Seyed Sadjadi- Senior ScientistTechnical TipsThe Column Doctor Is In
The Stages of Column Death!
Guest Author: Senior Scientist, Seyed Sadjadi. Congratulations! You are the proud new owner of a small stainless-steel tube packed with white sand, which will bring you fabulous results…if it lasts Continue Reading
Selecting the Right Column for Your Reversed Phase Method
Selecting the right column for your reversed phase method isn’t always easy. We wanted to help in the decision-making process by breaking down the different steps you’ll need to consider Continue Reading
What is HPLC and How Does it Work?
Guest author: Dr. Ramkumar Dhandapani Chromatography is coined from the Greek word chroma- which means color and -graphein which means to write. First recorded use of column chromatography can be Continue Reading
What the HILIC is HILIC?
Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.
Technical Tip: Peak Apex Flattening
Peak shape issues at the apex of a peak are typically related to detector saturation. This symptom can be observed as flattening and/or noise at the peak apex.
Technical Tip: Principle HPLC Conditions for LC/MS
High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.
Technical Tip: Fronting Peaks
Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.