Technical Tip: Engineering successful analytical methods using HILIC as an alternative retention mechanism Overview: This technical tip will outline hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) as an alternative retention mechanism that is particularly effective for LC-MS. Continue Reading
Guest Author – James Turner, Technical Specialist Solid phase extraction (SPE) is routinely selected as a sample preparation technique prior to HPLC and GC analysis. It provides clean-up, analyte pre-concentration, Continue Reading
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There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.
Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.
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