pH Archives - SCIENCE UNFILTERED

FeaturedTechnical Tips

pH – Why Is It Critical To Your Mobile Phase Method Development?

February 11, 2020February 11, 2020 Science UnfilteredLeave a comment

how pH can affect your mobile phase method development

Guest Author: Namrata Saxena, Technical Specialist As a chromatographer not many of us are aware of how critical pH is in your method development, sometimes it is even considered as Continue Reading

Tagged Acids,bases,ionization,Method Development,Mobile Phase,pH

FeaturedLiquid Chromatography (LC)Technical Tips

Mobile Phase Preparation – Steps to Take for Ideal Analysis

January 23, 2020March 3, 2020 Science UnfilteredLeave a comment

Guest Author – Technical Expert Genevieve Hodson Liquid Chromatography is reliant upon a accurately made mobile phase. Many chromatographic problems can be attributed to an incorrectly prepared mobile phase. Contamination Continue Reading

Tagged Chromatography,constant pH,Filtering,Mobile Phase,pH,reagents,regenerated cellulose

FeaturedScience News

Google Honors Danish Biochemist Responsible for the pH Scale – SPL Sørensen

May 29, 2018May 29, 2018 Science UnfilteredLeave a comment

Søren Peder Lauritz Sørensen or S.P.L. Sørensen, was a Danish biochemist who is most known for his introduction of the pH scale in 1909 while studying the effect of ion Continue Reading

Tagged acidic,acidity,alkaline,Aqueous,basic,Beer,biochemist,Biochemistry,Brewing,Carlsberg Laboratory,Chemistry,Denmark,Google,Google Doodle,ion concentration,lager,pH,pH scale,potential of hydrogen,protein analysis,Proteins,Sørensen,SPL Sørensen,Yeast

FeaturedForensics / ToxicologySample PreparationTechnical Tips

Solid Phase Extraction & In-Well Hydrolysis for Analyzing Abusive Drugs

April 26, 2018June 15, 2018 Science UnfilteredLeave a comment

solid phase extraction analyzing drugs of abuse

The increase of testing drugs of abuse radically defines the workload for many laboratories, requiring testing methods to be accurate, reliable, and quick, which is where In-Well Hydrolysis and SPE Continue Reading

Tagged 96 well plate,basic drugs of abuse,Dilute-and-Shoot,drug analysis,Drugs,Drugs of Abuse,Free Sample,Generic Drugs,Hydrolysis,In-Well Hydrolysis,pH,Plasma,sample matrices,Sample Prep,Sample Preparation,serum,Solid Phase Extraction,SPE,SPE sorbent,Strata-X,Strata-X-Drug B,Strata-X-Drug B Plus,Urine,urine analysis,urine laboratories,Whole Blood

FeaturedSample PreparationTechnical Tips

Utilizing Mobile Phase pH to Maximize Preparative Purification Efficacy

August 22, 2017August 23, 2017 Science UnfilteredLeave a comment

Preparative chromatography is a specialized subset of chromatography, where the goal is to purify a target analyte (or analytes in some cases) from a complex mixture of crude sample. The Continue Reading

Tagged Gemini,Gemini-NX,Mobile Phase,organo-silica,pH,Preparative chromatography,Sample Prep,Trifluoroacetic Acid

Liquid Chromatography (LC)Technical TipsUncategorized

What the HILIC is HILIC?

June 23, 2016December 9, 2016 Science Unfiltered1 Comment

Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.

Tagged Acetate,Acetonitrile,Amino,Ammonium,Ammonium Acetate,Ammonium Formate,Analysis,Analyte,Analytes,Analytical,Analytical Chemistry,Analytical Science,Aqueous,Ascorbic acid,Bilayer,Buffer,Buffers,Chemistry,Chromatogram,Chromatograms,Chromatographer,Chromatographers,Chromatography,CMP,Column,Column Chromatography,Columns,Cytidine Monophosphate,Detection,Diol,Diol Bonded,Folic Acid,Formate,GMP,Guanosine Monophosphate,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,HILIC,HPLC Column,Hydrophilic,Hydrophilic Interaction Liquid Chromatography,IMP,Injection,Injections,Inosine Monophosphate,LC,LC/MS/MS,Liquid,Liquid Chromatography,Liquid Chromatography (LC),Luna,Methanol,Mobile Phase,Monophosphate,Nicotinamide,Nicotinic acid,Organic,Organic Solvent,PABA,Peak,Peaks,Pentafluorophenyl,pH,Phenomenex,Pyridoxine,Reversed Phase,Riboflavin,Science,Sensitivity,Separation,Separation Sciences,Solvent,Solvents,Stationary Phase,Technical Tips,Thiamine,Thymidine Monophosphate,TMP,UHPLC,UMP,Unbonded Silica,Uridine Monophosphate,Vitamin B12,Water

Liquid Chromatography (LC)Science NewsUncategorized

The Proof is in the Litter: How an Innovative Product is Saving Feline Lives

March 23, 2016April 15, 2016 Science Unfiltered1 Comment

Many pet owners can tell if their furry friend is sick just by looking at them. What if there was a possible diagnosis lurking in your cat’s litter?

Tagged Absorb,Absorbent,Bile,Bile Duct,Bile Duct Obstruction,Bilirubin,Bio,Biochemistry,Biological,Biology,Cat,Cat Feces,Cat Food,Cat Litter,Cat Pee,Cat Poop,Cat Urine,Cats,Chlorophyll,Cholestasis,Chromatography,Color,Color-Based Technology,Color-Changing Technology,Column,Column Chromatography,Columns,Daniel Rotman,Diagnose,Diagnosis,Diagnostic,Dog Food,Feces,Feline Lower Urinary Tract Disease,FLUTD,Gallstones,Health,HPLC Column,Kidney,Kitten,Kittens,Kitties,Kitty,Kitty Litter,Liquid Chromatography (LC),Litter,Liver,Liver Inflammation,Melamine,Mikhail Tsvet,Mood Rings,Pancreatitis,Pee,Pet,Pet Food,Pet Litter,Pets,pH,Pigment,Pigmentation,Plant Pigments,Poop,Pregnancy Tests,Silica,Silica Gel,Stationary Phase,Struvite,Struvite Crystal,Struvite Crystal Formations,Struvite Crystals,Technology,Urine,Vet,Veterinarian,Veterinary

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Principle HPLC Conditions for LC/MS

January 18, 2016May 16, 2017 Science UnfilteredLeave a comment

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.