There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.
Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.
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In the spirit of frugality, process chromatographers often want to save and store their media for future use. There are steps one can take to store bulk resins for short term and long term use, though it’s worth noting that the media will not last forever.
Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.
The boiling point is the temperature where liquid has enough energy that molecules transferring into the vapor phase exert a pressure that is equal to the outside pressure. This allows molecules anywhere—not just the surface—to transfer to the gas phase.
In the first GC columns, gas chromatography phases were simply coated onto the column. This was mostly the case with packed columns, but also the same with the very first capillary columns. Injecting water onto these phases dissolved the phase and caused the phase to elute from the columns.
High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.
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