Best and Worst Organic Solvents to Use in LC

There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.

What the HILIC is HILIC?

Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic mode used mainly for the separation of polar compounds that are too hydrophilic to be retained in a reversed phase column.

10 Questions You’ve Always Wanted to Ask About LC

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Technical Tip: Bulk HPLC Sorbent Storage and Cleaning for Reuse

In the spirit of frugality, process chromatographers often want to save and store their media for future use. There are steps one can take to store bulk resins for short term and long term use, though it’s worth noting that the media will not last forever.

Technical Tip: Early Eluting Peak Shape

Depending on method conditions, early eluting compounds are apt to have poor peak shapes. Within are some steps that can be implemented to help minimize any distortions to early eluting peaks in gas chromatography.

How Does Temperature Affect a Compound’s Retention Time?

The boiling point is the temperature where liquid has enough energy that molecules transferring into the vapor phase exert a pressure that is equal to the outside pressure. This allows molecules anywhere—not just the surface—to transfer to the gas phase.

Technical Tip: Fronting Peaks

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Technical Tip: Choosing & Changing Syringe Wash Solvents

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.

Technical Tip: (BOO!) Ghost Peaks

Scientists aren’t perfect. Nobody is. In our Technical Tips, we can solve your most debilitating issues—from mechanics to methodology—together.