Doing HPLC Method Development for Monoclonal Antibody Aggregates? Wait a “SEC”!

By Guest Author: Brian Rivera Contrary to popular belief, size exclusion chromatography (SEC) isn’t straightforward. However, it should be—it’s a non-absorptive separation, right? Just look up an SEC column’s calibration curve, use a standard mobile phase like 1X PBS, and voila, done and done. Of course, that isn’t the case. Continue Reading

Optimizing Sample Preparation Methods for Comprehensive Drug Research Panels from Urine

Gluca-what? Hydrolysis? Beta-something? While the terminology for urine drug testing can be confusing, the methods should not be. Improve upon traditional urine testing by learning the science behind the steps and simplifying the sample preparation. When drugs are ingested, they are tagged with a glucuronic acid during the metabolism process, Continue Reading

What is USP Chapter 621?

The United States Pharmacopeia (USP) has defined the limits of “allowable adjustments” in USP General Chapter <621>, where revalidation is required if adjustment limits are exceeded. Even though this chapter might be taken as more “guidelines” than “rules”, it is best to heed the warning and take the precautions that Continue Reading

Selecting the Right Column for Your Reversed Phase Method

Selecting the right column for your reversed phase method isn’t always easy. We wanted to help in the decision-making process by breaking down the different steps you’ll need to consider when selecting your column. For a full in depth look into the selection process see our Ultimate Guide to HPLC/UHPLC Continue Reading

ADCs-Magic Bullets, Failed Drugs, and Heterobifunctional Linkers

Guest Author: Brian Rivera, Product Manager: Bio Separations Over a hundred years ago, Nobel Prize laureate, Paul Ehrlich, envisioned a drug which could kill microbes but not harm the body, a “magic bullet” he called it. From mustine to monoclonal antibodies, researchers have implemented Ehrlich’s side chain theory in attempts Continue Reading

Transform Your HPLC and UHPLC Method Development Mindset on Analyzing Proteins— Including Monoclonal Antibodies!

Method development and analyzing proteins

A discussion of wide-pore/core-shell technology for monoclonal antibodies, method development considerations with wide-pore/core-shell columns, size exclusion chromatography (SEC) using sub-2 μm particles, and method optimization for SEC. (Webinar sneak peek) Click Here To View Entire Webinar   Introduction Analyzing Proteins  Analytical chemists have relied on high performance liquid chromatography (HPLC) Continue Reading

SPE vs LLE: A Battle of Methods

An Improved Clean Up and Recovery of Pharmaceutical Compounds from Plasma using Strata® -X In one corner, we have liquid-liquid extraction (LLE), which is perhaps the most established clean-up technique used in the chromatography field. Although it has been used for years, newer techniques with improved specificity towards particular analytes, Continue Reading

What is Chiral?

Chiral chromatography pharmaceutical

What Exactly Does Chiral Mean? Chirality is a special physical property of a molecule which describes its geometry in three-dimensional space. Chiral molecules commonly consist of a carbon atom attached to four different substituents. The substituents can either be atoms or groups of atoms, but each must differ from the Continue Reading

First ALS Drug Approved in Over 2 Decades

A social media sensation has finally paid off. The U.S. Food and Drug Administration (FDA) has recently approved the first drug to combat amyotrophic lateral sclerosis, or ALS, in 22 years. The new drug, Radicava, has shown that it can slow progress of the physical conditions related to the disease by Continue Reading

Nobel Prize Winner Recognized Decades After Artemisinin Discovery

On October 5, 84-year-old Chinese scientist Tu Youyou earned the Nobel Prize for medicine, almost 50 years after she discovered artemisinin—otherwise known as “humankind’s best defense” against malaria.