Tag: <span>Flow Rate</span>

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How to Install a Flash Column onto an ISCO CombiFlash Rf Instrument

February 25, 2019February 25, 2019 Science UnfilteredLeave a comment

Steps to Properly Program Your Claricep Flash Column on an Rf Instrument Having trouble getting your flash column to connect with your ISCO CombiFlash® Rf Instrument? Keep getting the same Continue Reading

Tagged Chromatography,Claricep,Claricep Flash,Claricep Flash Column,CombiFlash Rf,flash chromatography,flash column,Flow Rate,ISCO CombiFlash Rf Instrument,REDISEP,RediSep Column,RediSep Rf Column,Rf Instrument

FeaturedLiquid Chromatography (LC)Pharma / Biopharma

Using Core-Shell Technology to Improve HPLC Methods within USP: Part 5

December 14, 2017December 14, 2017 Science UnfilteredLeave a comment

Guest Author: Dr. Jeff Layne Part V: Some Final Points on the Chromatographic Impact of Making Adjustments to Existing Methods We are finally coming to the end of our series, Continue Reading

Tagged Allowable Adjustments to USP Methods,Column Inner Diameter,Core-shell technology,European Pharmacopoeia,Flow Rate,Particle Size,US and Euro Pharmacopeia,USP Monographs

FeaturedLiquid Chromatography (LC)

The Effects of Changing Column Inner Diameter on LC-MS Applications-Part 3

August 15, 2017August 15, 2017 Science UnfilteredLeave a comment

Part 3: What is the Best Column ID for You? Hi, and welcome back to the third installation of this article series on the effect of changing column inner diameter (ID) Continue Reading

Tagged Applications,Chromatography,Column ID,Column Inner Diameter,Flow Rate,HPLC/UHPLC,LC Column,lc-ms

FeaturedLiquid Chromatography (LC)Technical Tips

The Effects of Changing Column Inner Diameter on LC-MS Applications- Part 2

July 27, 2017February 26, 2018 Science UnfilteredLeave a comment

Part 2: Changing Column Inner Diameter with Constant Flow Rate Guest Author: Dr. Jeff Layne Welcome back to this article series on the effects of changing column inner diameter on Continue Reading

Tagged Column ID,Column Inner Diameter,Flow Rate,Kinetex,LC/MS,SCIEX

FeaturedLiquid Chromatography (LC)

The Effects of Changing Column Inner Diameter on LC-MS Applications- Part 1

July 18, 2017August 9, 2017 Science UnfilteredLeave a comment

Part 1: Changing Column Inner Diameter While Adjusting Flow Rate Guest Author: Dr. Jeff Layne Welcome everyone! Jeff Layne here, back with another article series, and this time we are Continue Reading

Tagged Column ID,Column Inner Diameter,Flow Rate,HPLC,lc-ms

FeaturedLiquid Chromatography (LC)

Technical Tip: Column Loadability

May 23, 2017 Science UnfilteredLeave a comment

how much am I able to load on a reverse phase normal phase hplc column

Do you ever worry about your column’s loadability? Do you find yourself Googling, “How much am I able to load onto XYZ phase?” Ever fret over this issue so much Continue Reading

Tagged column loadability,elution time,Flow Rate,HPLC,loading capacity,Peak,retention time

Liquid Chromatography (LC)Technical TipsUncategorized

Best and Worst Organic Solvents to Use in LC

July 14, 2016July 14, 2016 Science Unfiltered1 Comment

There are several critical characteristics to take into consideration when choosing the appropriate organic solvent to use in liquid chromatography. For example, high viscosity solvents may produce backpressures that are too high for the HPLC system used.

Tagged Acetone,Acetonitrile,Analyte,Analytes,Analytical,Analytical Chemistry,Analytical Science,Backpressure,Backpressures,Best,Chemistry,Chromatogram,Chromatographer,Chromatographers,Chromatography,Column,Column Chromatography,Column Cleaning,Columns,Elute,Elution,Ethanol,Fatty,Flow,Flow Rate,High Backpressure,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,Highly Pigmented,HPLC Column,HPLC System,Iso Propanol,LC,LC/MS/MS,Liquid Chromatography,Liquid Chromatography (LC),Methanol,n-Propanol,Organic,Organic Solvent,Organic Solvents,Peak,Peaks,Pigment,Pigmented,Polarity,Polarity Index,Protein,Proteins,Regeneration,Science,Separation,Separation Sciences,Solubility,Solubilizer,Soluble,Solvent,Solvents,Technical Tips,Tetrahydrofuran,UHPLC,UV,UV Cutoff,UV Detection,UV/Vis,Viscosity,Worst

Fun ScienceHumanity in ScienceLiquid Chromatography (LC)Our CompanyUncategorized

Humanity in Science: Combating Counterfeit with Capillary Electrophoresis

April 26, 2016September 1, 2016 Science Unfiltered1 Comment

The Humanity in Science Award was created in 2015 to recognize outstanding developments in science that could change human lives for the better. There were many runners-up who did (and do!) amazing things for humanity. Recently, The Analytical Scientist sat down with each of them to catch up, and find out how their ventures have progressed in the last 12 months.

Tagged Africa,Analytical,Analytical Chemistry,Analytical Science,Antimalarial,Antimalarial Medication,Atom,Atoms,Award,Capacity,Capillary,Capillary Electrophoresis,CDC,CE,Centers for Disease Control and Prevention,Chromatography,Column,Columns,Cost,Counterfeit,Counterfeited,Detect,Detection,Developing Countries,Drug,Drugs,Electric,Electric Field,Electrokinetic,Electrokinetic Separation,Electrokinetic Separation Techniques,Electrophoresis,Electrophoretic,Electrophoretic Mobility,Emerging Countries,Evaluation,Fake,Feature,Flat Flow,Flow,Flow Rate,Fraud,Fraudulent,High Performance Liquid Chromatography,High Pressure Liquid Chromatography,Humanity in Science,Humanity in Science Award,Julie Schappler,Liquid Chromatography (LC),Malaria,Malarial,Medication,Medicine,Medicine Evaluation,Meds,Mobility,Parabolic Flow,Peak,Peak Capacity,Peaks,Pharma,Pharmaceutical,Pharmaceuticals,Pharmacology,Phenomenex,Philanthropy,Science,Separation Sciences,Serge Rudaz,Smartphone,Solvent,Spotlight,The Analytical Scientist,The Humanity in Science Award,UC Davis,UHPLC,WHO,World Health Organization

Liquid Chromatography (LC)Technical TipsUncategorized

Technical Tip: Principle HPLC Conditions for LC/MS

January 18, 2016May 16, 2017 Science UnfilteredLeave a comment

High-performance (or pressure) liquid chromatography, more commonly known as HPLC, can be a useful tool to assist in the improvement or simplicity of mass spectral detection and analysis.